HPTLC

this presentation presents introduction about high performance thin layer chromatography, its features, principle and instrumentation along with its applications. it also gives comparison between TLC and HPTLC. instrumentation is given in a sequence for easier understanding of instrument.

1. HPTLC S. PREETHI I M.Sc FSN

2. High Performance Thin Layer Chromatography (HPTLC). Recent development led to the improvements in various operations involved. Difference b/w TLC & HPTLC – practical technique than of the physical phenomenon (partition, adsorption) on which separation is based. INTRODUCTION

3. INTRODUCTION

4. PRINCIPLE Similar approach & same physical properties of TLC. Principle of separation – adsorption. Mobile phase flows through b/c of capillary action. Components move according to their affinities towards adsorbent. Components – separated on chromatographic plate.

5. PRINCIPLE

6. INSTRUMENTATION

7. INSTRUMENTATION

8. 1. SAMPLE PREPARATION

9. 2. CHOMATOGRAPHIC LAYERS

10. 3. SAMPLE APPLICATION

11. 4. Pre - conditioning Low polarity of mobile phase – no need for saturation. High polarity of mobile phase – saturation is needed. 5. MOBILE PHASE Solution by trial & error where chemical properties of solute & solvent solubility of analytic adsorbent layer are considered.

12. Linear development method. plate – vertically. placed in solvent system in a suitable container. solvent – fed by capillary action & chromatogram – from both the sides. 6. CHROMATOGRAPHIC DEVELOPMENT

13. 7. DETECTION SPOTS Immediately after the development is completed, the plates are removed from the chamber and dried to remove the frees of mobile phase. D etection - known by iodine vapor in iodine chamber.

14. 8. SCANNING & DOCUMENTATION

15. FEATURES

16. APPLICATION

17. THANK YOU