In situ hybridization

In situ hybridization

1. Presented by Neelma Nayab Biotechnology and GE Mphil 2 nd semester kust

2. In situ hybridization In situ hybridization (ISH) is a powerful technique for localizing specific nucleic acid targets within fixed tissues and cells, allowing you to obtain information about gene expression and genetic loci.

3. Type of ISH Fluorescence (FISH) Chromogenic (CISH) detection

4. Fluorescence in situ hybridization fluorescence in situ hybridization is a molecular cytogenic technique that uses fluorescence probes that binds to only those parts of the chromosomes with a high degree of sequence complementarity . It is used to detect and localize the presence or  absence of specific DNA sequences on  chromosomes.

5. Chromogenic in situ hybridization Chromogenic in situ hybridization (CISH) enables you to gain genetic information in the context of tissue morphology

6. Probes A probe is defined as a single stranded piece of DNA, labelled (either with radioisotope or with non-radioactive label), the nucleotide sequence of which is complementary to the target DNA

7. Types of probes

8. Application of ISH