Industrial production, estimation and utilization of phytoconstituents

Industrial production, estimation and utilization of phytoconstituents

1. INDUSTRIAL PRODUCTION, ESTIMATION AND UTILIZATION OF PHYTOCONSTITUENTS Presented By: Mahewash Sana A. Pathan. Department of Pharmaceutics , R. P. College of Pharmacy, Osmanabad .

2. FORSKOLIN Biological Source : Labdane diterpenoid extracted from roots of Coleus forskohlii , family- Lamiaceae . 2

3. Industrial Production: 3 FORSKOLIN

4. Estimation: TLC & HPTLC Mobile phase – Toluene: ethyl acetate ( 8.5: 1.5 v/v) Stationary phase- Silica gel F254 Visualizing agent- 5% vanillin in glacial acetic acid and 10% sulphuric acid in water. Utilization: Antidepressant Vasodilating Antiobesity In glaucoma A ntiasthmatic 4 FORSKOLIN

5. SENNOSIDES Source: Dianthrone glycosides, leaflets of Cassia angustifolia (Indian senna ) & C. acutifolia ( Alexandrian senna ). Family- Leguminosae . 5

6. Industrial production: 6 SENNOSIDES

7. Estimation: Column- C18 Mobile phase- 1% acetic acid in water: Acetonitrile (82:18) Flow rate- 1ml/min Detection- 350 nm Utilization: Treatment of constipation In skin diseases As an anthelmintic Useful in loss of appetite, dysentry, indigestion, malaria, jaundice, gout, rheumatism & anaemia. 7 SENNOSIDES

8. ARTEMISININ Source: sesquiterpene lactone obtained from the leaves & unexpanded flower heads of Artemisia annua . Family- Asteraceae . 8

9. Industrial production: 9 ARTEMISININ

10. Estimation: HPLC & HPTLC method Mobile phase- n-hexane : ethyl acetate ( 7.5: 2.5 v/v) Stationary phase- silica gel F254 Visulazing agent- anisaldehyde sulphuric acid reagent followed by heating to 110 ˚C. Utilization: Antimalarial In gastric infections Suppress inflamatory immune reactions Anticancer 10 ARTEMISININ

11. DIOSGENIN Source: Aglycone obtained after the hydrolysis of steroidal saponin glycoside dioscin present in Dioscorea deltoidea , D. composite. Family- Dioscoreaceae . 11

12. Industrial production: 12 DIOSGENIN

13. Estimation: HPTLC method Mob. Phase- toluene: ethyl acetate: formic acid (5:4:1) St. phase- Silica gel F 254 Utilization: As a precursor for steroidal synthesis In preparation of oral contraceptives In treatment of rheumatism. 13 DIOSGENIN

14. DIGOXIN Source: Cardiac glycoside obtained from leaves of Digitalis lanata . Family- Scrophulariaceae . 14

15. Industrial production: 15 DIGOXIN

16. DIGOXIN Estimation: Assay- 40 mg test & std solution of digoxin dissolve in sufficient ethanol. 5 ml of resulting solution, add 3ml picric acid solution. Measure absorbance at 495 nm. Utilization: treatment of cardiac disorders. 16

17. ATROPINE Source: tropane alkaloid, flowering tops of Atropa belladonna, Datura stramonium & Hyoscyamus niger . Family- Solanaceae . 17

18. ATROPINE Industrial production : 18

19. Estimation: Assay- sulphate salt of atropine titrated against 0.1 N perchloric acid. Utilization: As preanesthetic medication Antispasmodic 19 ATROPINE

20. PODOPHYLLOTOXIN Source: resin, roots & rhizomes of Podophyllum hexandrum , P. emodi & P. peltatum . Family- Berberidaceae . 20

21. PODOPHYLLOTOXIN Industrial production: 21

22. Estimation: HPLC Mob. Phase- methanol: water ( 62: 38 v/v) Detector wavelength- 280nm. Utilization: Antitumour Purgative Emetic Treatment of warts 22 PODOPHYLLOTOXIN

23. CAFFEINE Source: xanthine alkaloid, leaves of Camellia sinesis ( Theaceae ), seeds of Coffea arabica ( Rubiaceae ). 23

24. CAFFEINE Production: 24

25. Estimation: HPLC method Mob. Phase- methanol: acetonitrile ( 65: 35 v/v) Column- C18 Utilization: Stimulant 25 CAFFEINE

26. TAXOL Source: nitrogen containing subs, bark of Taxus brevifolia , fam - taxaceae . 26

27. TAXOL Production : 27

28. Estimation: HPTLC method Mob phase- chloroform:methanol (7:1v/v) Visualizing agent- vanillin sulphuric acid. Utilization: Treatment of ovarian, lung, bladder, esophageal & other types of cancers. Antiproliferative agent. 28 TAXOL

29. VINCRISTINE & VINBLASTINE Source: Indole alkaloid, Vica rosea , family- Apocynaceae . 29

30. VINCRISTINE & VINBLASTINE Production: Plant tissue culture technique. Estimation: HPLC method Mob phase- acetonitrile : 0.1 M phosphate buffer. Wavelength- 254nm. Utilization: In chemotherapy regimens Childhood leukemia immunosuppressant 30

31. THANK YOU 31