Introduction of Recombinant Antibody

Recombinant antibodies are antibody fragments generated by using recombinant antibody coding genes as a source and display technology, delivering high reproducibility, specificity and scalability. Unlike monoclonal antibodies (mAbs) which are produced using traditional hybridoma technologies, rAbs do not need hybridomas and animals in the production process if you only use synthetic genes.

1. Introduction of Recombinant antibody www.creative-biolabs.com

2. Conventional Antiboby Antibody ; Immunoglobulins Paratope of antibody Plasma cells

3. Antibody Structure Be composed of: Two heavy chains Two light chains Two structural region: Constant region Variable region

4. Five Isotypes of Antibody Antibody Isotype Type of Heavy Chain Sub- isotypes Structure Function IgA α IgA1 , IgA2 Monomer Tetramer Preventing pathogens invading into tissues from epiderm IgD δ N/A Monomer Activating basophils and mast cells to produce antimicrobial factors IgE ε N/A Monomer Interacting with allergens, facilitating histamine releasing from mast cells and basophils , preventing worms infection IgG γ IgG1, IgG2, IgG3, IgG4 Monomer The major antibody fighting against pathogens IgM μ N/A Monomer Pentamer Provide early elimination of pathogens before the generation of sufficient IgG

5. Antibody Isotypes Predominant in secretions Monomeric form in blood IgA IgD Function is unclear Receptor on B cells IgG 4 subclasses due to variation in γ serum concentration: IgG1>IgG2>IgG3>IgG4 IgE Bound to surface of mast cells/ basophils Most efficient at antigen-binding Monomeric IgM as mIgM on B cells Joined by J chain IgM

6. Recombinant Antibody Production B cell repertoire Antibody gene Gene Clone Recombinant DNA Phage display Yeast display Ribosome display Recombinant antibody Fab fragment ScFv Single domain antibody ( SdAb )

7. Three Common Recombinant Antibody Fragment VH , VL and linker w ithout ch1 Camelid antibody Shark antibody Single domain antibody( sdAb ) scFv Fab Papain

8. Advantage & Disadvantage of Recombinant Antibody Recombinant Antibody Smaller size m onovalency ; Ease of engineering & manufacture; Improved tissue penetration; Lack of potentially deleterious Fc effector function. Conventional Antibody Lower antibody yield; Highly training & experienced technician.

9. Recombinant Antibody Provided by Creative Biolabs Products : scFv Fragment Antibodies Fab Fragment Antibodies Single Domain Antibodies Rabbit Monoclonal Antibodies Services : Recombinant Antibody Construction Recombinant Antibody Purification & Expression

10. Address: 45 -1 Ramsey Road, Shirley, NY 11967, USA Tel: [phone] Fax: [phone] Email: [email protected] Web : www.creative-biolabs.com Contact Us for Recombinant Antibody