Introduction to biosynthetic pathway

Unit 1 of Pharmacognosy and phytochemistry II V semester Third year pharmacy

1. Prepared by
Kulkarni D.M.
Asst. Professor,
Yash Institute of Pharmacy,
Aurangabad
Biosynthetic Studies &
Basic Metabolic Pathways

2. WhatisBiosynthesis?
●Biosynthesis is a process of forming larger organic
compounds from small subunits within a living organism.
Biosynthesis is mainly done by enzymes.
●Biosynthesis is also known as anabolism since simple
compounds are joined together to form macromolecules by
enzymes.
●As an example, photosynthesis occurs inside the chloroplast.
●The light energy is converted into chemical energy during
photosynthesis.
●The larger molecule glucose is biosynthesized from water and
carbon dioxide by photosynthetic organisms.(ATP, Enzyme,
Cofactors)

4. Difference Between Synthesis and Biosynthesis?
Synthesisvs.Biosynthesis
Synthesis refers to the formation of
macromolecules from small
molecules artificially.
Biosynthesis refers to the formation
of larger organic compounds from
small molecules within a living
organism.
Process
Synthesis is artificial and chemical. Biosynthesis is biological and
catalyzed by enzymes.
Resulting Polymers
Synthesis can result in polymers
which are organic or non-organic.
Biosynthesis is biological and
catalyzed by enzymes.
Occurrence
Synthesis occurs outside living
organisms.
Biosynthesis occurs within a living
organism.

5. Biosynthesis of Primary
Metabolites

Livingplantsare solar-poweredbiochemical and
biosynthetic laboratory whichmanufactures bothprimary
andsecondary metabolitesfrom air,water, minerals
andsunlight.


The primary metabolites like sugars, amino acids & fatty acids
that are needed for general growth & physiological development of
plant which distributed in nature & also utilized as food by man.


The secondary metabolites such as alkaloids, glycosides,
Flavonoids, volatile oils etc are biosyntheticallyderived
from primarymetabolites.


Biosynthetic reactions are replica of common organic reactions
like catalytic reactions, phosphorylation, hydride transfer, oxidation,
elimination, acylation, alkylation, reduction, condensation,
rearrangement etc.

6. Metabolism & Metabolic
Pathways
●Cell Metabolism: Process by which living cell
process nutrient molecule & living state.
●Metabolic Pathway: A complete set of chemical
reactions that occur in living cells, allowing cells
to grow and reproduce, maintain their structures,
and respond to their environments.
●Living cell require energy for biosynthesis,
transport of nutrient, motility and maintenance.
●Energy is obtained from the catabolism of
carbon compounds (carbohydrate)
●Carbohydrates are synthesized from CO
2
and H
2
O
in the present of light by photosynthesis.

7. ~ produce energy to the cell
~ requires energy
glucose to glycogen

8. Major Metabolic Pathways
●Cellular respiration:
Glycolysis
Anaerobic respiration
Kreb’s cycle / Citric acid cycle Oxidative
phosphorylation
●Creation of energetic compounds from non-living matter:
Photosynthesis (plants, algae cynobacteria)
Chemosynthesis (some bacteria)
Other pathways occurring in (most or) all living cell: Fatty
acid oxidation (β-oxidation)
Gluconeogenesis
HMG-CoA reductase pathway (isoprene prenylation) Pentose
phosphate pathway (hexose monophosphate) Porphyrin
synthesis (or heme synthesis) pathway
Urea cycle

9. Metabolites
●Metabolites are the intermediates & products of
metabolism.
●The term metabolite is usually restricted to small
molecules.
●A primary metabolite is directly involved in the
normal growth, development, and reproduction.
●A secondary metabolite is not directly involved
in those processes, but usually has important
ecological function.

10. Importance of photosynthesis in
formation of primary metabolites
●Photosynthesisistheprocesswhereplantsconvertsunlightinto
energy,thenstoreitas carbohydrates,sugars,suchasglucose.
●Photosynthesismay bethemost important processin
ecosystems, bothbringsinenergy needed withinthe
ecosystem,and produceoxygen (O
2
) neededforcellular
respiration,andthe production of more ATP.

●Photosynthesis has three basic steps:
1.Energy is captured from the sunlight.
2.Light energy is converted into chemical energy in the form of ATP and NADPH.
3.Chemical energyisusedtopowerthesynthesisoforganic
molecules(e.g.carbohydrates) from carbon dioxide (CO
2
).

11. Photosynthesis
●H
2
O + light + ADP + P ---> O
2
+ ATP + e-
●After the above steps occur in photosystem II,
the electron is finally sent to photosystem I,
where the following happens.
●e- + NADP
+
+ H ---> NADPH
●Now there are two high energy molecules,
fully charged and ready to be used. Plants
make more energy that it needs immediately,
so the NADPH and ATP are used to make
glucose as follows:
●CO
2
+ ATP + NADPH ---> C
6
H
12
O
6
●This happens in Calvin cycle.

12. Theprimaryandsecondary metabolites
derivedfromcarbonmetabolism

14. difference between Primary and
secondary metabolites