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Isolation and characterization of fungi

1. ISOLATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH SOLID WASTE IN UNIVERSITY OF MAIDUGURI CAMPUS PRESENTED BY: Babangida Adamu I.D NUMBER: 18/08/06/ 2 3 3 SUPERVISED BY: MISS GRACE A. FELIX A P R O J ECT P R O P O SAL D E F E N S E PRESENT E D TO THE DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCE, UNIVERSITY OF MAIDUG URI , B ORNO STATE. AUGUST , 2023 .

2. I N T RODUCTION Solid waste management is a global challenge that has significant health and environmental implications. Fungi are known to be present in solid waste and can cause a variety of problems, including the production of toxins, the degradation of materials, and the release of harmful compounds into the environment. In addition, the presence of fungi in solid waste can create an unsanitary environment that can lead to the spread of diseases. The University of Maiduguri campus is not immune to these problems, as the presence of solid waste has been identified as a major challenge. ( Abegaz, A. A. 2007 )

3. S T ATEMENT OF PROBLEMS The University of Maiduguri campus has a significant solid waste management challenge, which may have an impact on public health and environmental quality. The presence of fungi associated with solid waste may be a significant contributor to this problem. However, there is limited information on the specific types of fungi that are present in the campus' solid waste, as well as the characteristics of these fungi. Therefore, there is a need to identify and characterize the fungi associated with solid waste in the University of Maiduguri campus, to better understand the extent of the problem and develop appropriate solutions.

4. J USTIFICATION OF STUDY The isolation and characterization of fungi associated with solid waste in the University of Maiduguri campus is important for several reasons. First, it will help to determine the specific fungi that are present in the campus' solid waste, which is essential for understanding the extent of the problem and developing appropriate solutions. Second, it will provide information on the characteristics of these fungi, such as their growth rate, nutritional requirements, and ability to produce toxins, which is important for assessing their potential impact on public health and the environment.

5. AIM S The aim of this study is to isolate and characterize the fungi that are associated with solid waste in the University of Maiduguri campus, in order to better understand their potential impact on public health and the environment.

6. OBJECTIVES - Determine the types of fungi present in solid waste collected from the University of Maiduguri campus.
- Identify the morphological characteristics of the fungi isolated from solid waste.
- Assess the antimicrobial activity of the isolated fungi.
- Assess the heavy metal content of the fungi isolated from solid waste.

7. MATERIALS Sterile C o n i c a l F l a s k s S t e r i l i zed t r o w e l S t e r i l e p e t r i d i s h I n c u b a tor C u l t u r e media Biochemical i d e n t ification t e c h n iques D i s t i l l ed w a t e r P henolphthalein indicator E . T . C

8. M ETHODS The methods for this study will include the collection and analysis of solid waste samples from the University of Maiduguri campus. A total of 10 solid waste samples will be collected from various locations on the campus, such as residential halls, the library, and the cafeteria. The samples will be placed in sterile bags and transported to the laboratory for analysis. The samples will be processed using standard microbiological techniques, such as serial dilutions and plating on agar media. Fungal colonies will be identified based on colony morphology, microscopy, and molecular techniques. The results will be analyzed using statistical methods.

9. EXPECTED OUTCOME The expected outcome of the study is to gain a better understanding of the fungi that are associated with solid waste in the University of Maiduguri campus. It is anticipated that the study will identify the specific fungi that are present, as well as their characteristics and potential health and environmental impacts. It is hoped that this information will lead to the development of effective strategies for the management of solid waste in the campus, and the prevention of any associated health and environmental risks.

10. REFERENCES Abegaz, A. A. (2007). Assessment of solid waste management practices and their environmental and health impacts in Maiduguri, Nigeria. International Journal of Environmental Science and Technology, 4(2), 135-145. doi: 10.1504/IJEST.2007.013742 Ikram-Ul-Haq, M., Khan, S., Safdar, M., Javed, S., Tariq, M., & Farooq, U. (2015). Isolation and identification of fungi from solid waste dumps. Pakistan Journal of Botany, 47(5), 1963-1971. Wentao, Y., Yonghui, L., Xiaolin, Y., Jie, L., Yixue, Z., & Yi, T. (2014). Isolation, characterization and evaluation of antimicrobial activity of endophytic fungi from cucumber and their host plants. International Journal of Biological Sciences, 10(4), 323-330. Lawal, S. O., Deyor, A. B., & Oladosu, S. B. (2014). Microbiological assessment of the bacteria and fungi in municipal solid waste dumpsite soils. African Journal of Environmental Science and Technology, 8(6), 430-440. Sivaram, P., & Kalaivani, G. (2018). Isolation and characterization of fungi from food wastes and their antifungal activity. Journal of Advanced Research in Microbiology and Biotechnology, 1(2), 50-53. doi: 10.5281/zenodo.2582135

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