ISOLATION OF potential ALGAE FROM SOIL.pptx

Isolation of algae from soil

1. ISOLATION OF ALGAE FROM SOIL Presented by: Shamily I MSc Microbiology Sri Paramakalyani college Alwarkurichi

2. CONTENTS • INTRODUCTION • HABITAT • ISOLATION - Procedure - Observation

3. INTRODUCTION Algae are Eukaryotic Phototrophic organisms, requiring light for photosynthesis.There are atleast 20,000 identified species of algae. They may be unicellular or multicellular. Based on their size , they are classified into two forms; • Microalgae Eg : Chlamydomonas,Chlorella • Macroalgae Eg : Sargassum,Porphyra Soil and water is the most important source for isolation of algae.

4. HABITAT Algae are probably best known as the dominant plants in most aquatic habitats, Both freshwater & marine water.

5. While one generally thinks of algae as aquatic organisms , many are in fact adapted to living in dry terrestrial habitats ; including soil, the surfaces of rocks, even in desert sand. Most soil types contain rich communities of algal species.

6. Types of organisms found in soil Archae Actinomycetes Fungi Bacteria Algae Protozoa

7. The number of algae in soil usually ranges from 100 – 10,000 per gram of soil,their number decreases in deep soil, blue – green algae and diatoms are rich in soil. ISOLATION OF ALGAE FROM SOIL Surface or Subterranean soil samples are collected from different places. Sample collection :

8. Media employed : For isolation : Chu’s media / Beneck’s media COMPOSITION OF BENECK’S BROTH MEDIA COMPOSITION OF CHU’S MEDIA

9. • 1 gm of soil sample dissolved in 10ml of basal media (Stock Solution). MISSION • Required media for isolation is prepared , Poured into conical flasks and autoclaved. PROCEDURE : • 1ml of stock solution is serially diluted into successive test tubes containing 9ml of sterile water each. • 1 ml aliquots of each dilution is transferred to labeled conical flasks containing sterile media respectively.

10. SERVICE 01 SERVICE 02 SERVICE 03 SERVICE 04 • Once after the incubation, algae can be identified and Characterized using Microscope. • The flasks are kept for incubation near the source of bright light for 20- 30 days. • Individual Clonal populations / Colonies can then be obtained by Unialgal Culture techniques.

11. OBSERVATION : A drop of culture from incubated medium is transferred onto the microscopic slide and observed under low power microscope,the cells can be identified and separated for axenic culture establishment. Algal preparation can even be stained using general strain safranin for few minutes followed by it’s mounting using 10% glycerin. [ E.g., of stains: Aniline – blue, fast green, acetocarmine, haematotoxin etc., ]

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