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1. Bacteriology BCQs

2. Protein particles which can infect are called a. Virions b. Prions c. Nucleoida d. None of these

3. True statement regarding Endotoxin is a. Peptidoglycan b . Lippolysacharide c. Teichoic acid d. Inner membrane e. produced by G+ve bacteria

4. Which bacteria produces typical fried egg appearance colonies on solid media ? a. Mycobacteria b. Mycoplasts c. Mycoplasma d . Bacteroides e. E. Coli

5. Which of following is obligate anaerobic bacteria a. Streptococci pyogen b.E.Coli c.Pseudomonas d. Peptococcus e.Bacillus anthracis

6. Which of following is obligate aerobic bacteria a. Streptococci pyogen b.E.Coli c.Peptostreptococcus d. Peptococcus e.Bacillus anthracis

7. which of following is Non-Lactose fermenting G- ve Bacilli a. S.pyogen b. Enterobacter c. E.Coli d. Salmonella e. Klebseilla

8. which of following is Lactose fermenting G- ve Bacilli a. Lactobacillus b. Peptococcus c. E.Coli d.Salmonella e. Shigella

9. Which of the following is motile bacteria a. All streptococci b. All Zoonotics c. All enterobacteriacae except E.Coli d. All enterobacteriacae except klebsiella & shigella e. All enterobacteriacae except Proteus & providentia

10. Which of the following is non- mmotile bacteria a. All streptococci b. All Clostridia bacteria c. All spirochetes d. All enterobacteriacae except klebsiella & shigella e. Pseudomonas & vibrio cholera

11. Which of the following is Non-capsulated bacteria a. Klebsellia b. E.Coli c. Bacillus anthracis d. C.tetani e. All zoonotics

12. Which of the following is capsulated bacteria a. Proteus b. E.Coli c. Bacillus cereus d. Mycoplasma e. Spirochetes

13. Which of the following is Alpha hemolytic bile soluble Streptococci a. S.pyogen b. GBS c. S.pneomniae d. S.Virridians e. Sbovis

14. Which of the following is Alpha hemolytic optochin resistant Streptococci a. S.pyogen b. GBS c. S.pneomniae d. S.Virridians e. Sbovis

15. Which of the following is beta hemolytic bacitracin sensitive Streptococci a. S.pyogen b. GBS c. S.pneomniae d. S.Virridians e. Sbovis

16. Which of the following is beta hemolytic bacitracin resistant Streptococci a. S.pyogen b. GBS c. S.pneomniae d. S.Virridians e. Sbovis

17. Which of the following is Non- hemolytic & mannitol + ve Streptococci a. S.pyogen b. GBS c. S.pneomniae d. Enterofacalis e. Sbovis

18. Which of the following is beta hemolytic mannitol - ve Streptococci a. S.pyogen b. GBS c. S.pneomniae d. E.facalis e. Sbovis

19. Sterilization is usually carried out by Autoclaving b. Radiation c. filtration d. Ethylene Oxide e.Formaline

20. Sterilization consists of exposure to a. steam at 141 °C & pressure of 15 lb /in2 for 15 minutes b. steam at 141°C & pressure of 20 lb /in2 for 15 minutes c. steam at 1oo°C & pressure of 15 lb /in2 for 15 minutes d. steam at 121°C & pressure of 15 lb /in2 for 15 minutes e. steam at 200°C & pressure of 10 lb /in2 for 50 minutes

21. intravenous solutions are sterilized by a. autoclaving b. Radiation c. filtration d. Ethylene Oxide e.Formaline

22. Ethanol often used as an antiseptic to clean the skin prior to venipuncture a. 100% ethanol b. 90 % ethanol c. 70% ethanol d. 50% ethanol e. 40% ethanol

23. Glass & rubber made Surgical instruments are sterilized by a. autoclaving b. Filtration c. 70% ethanol d.Radiation e. ethylene oxide gas

24. Disinfect surgical site prior to surgery a.Iodophor b. Chlorhexidine c. 70% ethanol d. hydrogen peroxide e. Formaldehyde

25. Disinfect skin prior to venipuncture or immunization a.Gluteraldehyde b. Chlorhexidine c. 70% ethanol d. hydrogen peroxide e. Formaldehyde

26. Sterlization of surgical gowns& drapes is done by a. UV Radiation b. Autoclave c. 70% ethanol d. hydrogen peroxide e. Formaldehyde

27. Cleansing wounds is done by a. UV Radiation b. Steam c. 70% ethanol d. hydrogen peroxide e. Formaldehyde

28. Disinfectant of floor of operating room a. UV Radiation b. Steam c. 70% ethanol d. hydrogen peroxide e. Benzalkonium chloride

29. Cleansing of burn wounds is carried out by a. Chlorhexidine b. Silver sulfadiazine c. 70% ethanol d. hydrogen peroxide e. Formaldehyde

30. Sterlization of endoscopes , respiratory therapy equipments is done by a. UV Radiation b. Steam c. 70% ethanol d. Ethylene oxide / glutaraldehyde e. Hydrogen peroxide

31. Preservative used in vaccines a. Thimerosal b. Steam c. 70% ethanol d. Ethylene oxide e. Hydrogen peroxide

32. Staining BCQs

33. 12. In H&E staining a. Haemtoxyline stains cytoplasm b. Eosin stains Nucleus c. Cytoplasm appears Pink d . Nucleus appears Pink e. Nucleus appears Green

34. If only one stain is used for staining specimen Simple staining b. Negative staining c. Natural staining d. Differential staining e. Progressive staining

35. Which of the following is Basic stain/dye a. Eosin b. Crystal violet c. Giemsa d . Negrocin e. Indian ink

36. Which of the following is Acidic stain/dye a. Hematoxyline b. Crystal violet c. Giemsa d . Negrocin e. safranin

37. Which of the following is a neutral stain? a. Negrocin b . Giemsa c. Indian ink d . Crystal violet e. Safranin

38. Which of the following is progressive staining a. Gram’s staining b. ZN staining c. Acid fast staining d . Simple staining e. H&E

39. If other than the object the remaining portion is stained then it is called a. Simple staining b. Negative staining c. Positive staining d . Compound staining e. Progressive staining

40. If object is stained rather the remaining portion it is called a. Simple staining b. Negative staining c. Positive staining d . Compound staining e. Progressive staining

41. If more than one stain is used that staining is called a. Simple staining b. Compound staining c . Differential staining d . Negative staining e. Regressive staining

42. Which of the following is a neutral stain? a. Negrocin b . Giemsa c. Indian ink d . Crystal violet e. Safranin

43. Mordant used in Gram’s staining is safranin Heat Crystal Violet alcohol iodine

44. Mordant used in ZN staining is Malachite green Heat Crystal Violet alcohol iodine

45. cold method of ZN staining Done on low heat concentration of carbol fuchsin increased concentration of alcohol increased Slide is refrigereted Iodine is added

46. in ZN staining Malachite green is primary stain Cold method is known as Kinyoun stain carbol fuchsin gives blue colour alcohol is used as decolorizer iodine is mordant

47. Which is primary stain in ZN staining Crystal violet Eosin carbol fuchsin alcohal iodine

48. Mycoplasmas are bacterial cells that a. Penicillins cause frequent reaction with mycoplasma b. Have a rigid cell wall c .. h ave thin walled d. Stain well with Gram’s stain e. Are resistant to penicillin

49. The bacterial cells are at their metabolic peak during a. Lag phase b. Log c. Stationary d. Decline

50. Culture media

51. Identify the picture ?? Alpha ( incomplete) hemolysis on Blood agar

52. Identify the picture ?? Beta (Complete) hemolysis on Blood agar

53. Identify the picture Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Chocolate agar

54. Macconkey’s agar Salmonella Shegilla Klebsiella , E. Coli , Enterobacter Proteus , Proviodentia

55. Identify the picture Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Chocolate agar

56. Identify the picture Nutrient Agar TSI agr Blood Agar MacConkey's agar Chocolate agar

57. Identify the picture Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Chocolate agar

58. Identify the picture Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Chocolate agar

59. Bacteria produces yellow colonies in MacConkey’s agar Klebsiella E.coli Enterobacter S.pyogen Salmonella

60. Which one of the following is liquid culture media a. blood agar b. chocolate agar C. nutrient agar D. Nutrient Broth E. MacConkey’s Agar

61. Which of the following is Basic media Serum Agar Chocolate Agar Nutrient broth TSI Agar EMB Agar

62. BCQ Which of the following is Enriched media Nutrient Agar Nutrient broth TSI Agar Blood Agar Peptone water

63. BCQ Which of the following is Selective media Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Egg yolk medium

64. BCQ Which of the following is Differential Selective media Blood agar Chocolate agar Nutrient Agar Crystal Violet blood agar Serum agar

65. Agar is Liquefying agent B. Melting point is > 90 C. C. Polypeptide in nature D. Bacteria metabolize it easily E . In Nutrient Broth is >1.5%

66. Which of the following is Selective media Nutrient Agar Nutrient broth Blood Agar MacConkey's agar Egg yolk medium

67. Which of the following is Differential Selective media Blood agar Chocolate agar Nutrient Agar Crystal Violet blood agar Serum agar

68. Which of the following is transport media a . Mackonkey’s agar b. TSI agar c. Serum agar d . Amie’s medium e. EMB agar

69. Which of the following culture media is used to observe motility a . Mackonkey’s agar b. TSI agar c. Serum agar d . SIM media e. EMB agar

70. Peptone water medium is an example for a. Differential media b. Selective medium c. Basic culture media medium d. Selective & differential media

71. Special Bacteriology BCQs

72. MS ( mannitol salt) agar a. is basic media b. Supports S.pyogen c. Differntiates S.aureus & S.epidermis d . Differntiates S.pyogen & S.agalactiae e. Differntiates S.pneumoniae & S.viridants

73. Loffler's Medium Used to culture Streptococci G-ve Bacilli are cultured Corrynebacterium Dipthriae are cultured Metachromatic granules appear Green Cytoplasm appears Yellow

74. alpha hemolytic optochin sensitive bacteria is a. S.aureus b. S.pyogen c. S.peumoniae d . S. Viridians e. S.epidermis

75. alpha hemolytic optochin resistant bacteria is a. S. Viridians b. GAS c. GBS d . S.epidermis e . S.peumoniae

76. TCBS (Thiosulfate Citrate Bile Salt Agar ) a. Cultivates corona virus b. Cultivates Chlymadia c. Cultivates Mycobacteria d . Cultivates Vibrios e. Cultivates Mycoplasma

77. Lowenstein jensen ( LJ ) medium a. Cultivates Streptococci b. Cultivates Chlymadia c. Cultivates Mycobacteria d . Cultivates Vibrios e. Cultivates Mycoplasma

78. Cooked meat medium is used to grow a. Pseudomonas b . Bacillus anthracis c. Cultivates Mycobacteria d . anaerobes e. Chlymadia & Ricketsia

79. Which inhibitors are used in MacConkey’s agar to inhibit G+ve growth Crystal viloet Bile salts Neutral red Lactose A & B

80. color indicator used in MacConkey’s agar Litmus paper Neutral red Phenol red malachite green crystal violet

81. color indicator used in TSI agar is Litmus paper Neutral red Phenol red malachite green crystal violet

82. TSI agar Stands for Tri-Sucrose indole agar Sucrose is 10% Glucose is 1% alkaline colonies appear Green Acidic colonies appear Yellow

83. MacConkey’s agar is basic culture media used to culture anaerobic bactria Phenol Red is colour indicator alkaline colonies appear Black Acidic colonies appear Red

84. MacConkey’s agar Differentiates alpha & beta hemolytic bacteria Differentiates aerobic & anaerobic bacteria inhibits lactose fermenting bacteria supports anaerobic growth is selective & differential culture media

85. Bacteria produces Green -brown colonies in MacConkey’s agar Pseudomonas Salmonella proteus providentia E.coli

86. Slant in TSI agar Provides anaerobic environment Supports G+ve bacteria Support aerobic bacteria Is Black colour normally Green colour when E.coli grows

87. Butt in TSI agar Provides anaerobic environment Supports G+ve bacteria provides aerobic environment Becomes white when salmonella grows Becomes Green when salmonella grows

88. in TSI agar lactose fermenting bacteria appears as Red slant & Red butt Black slant & Red Butt Yellow slant & Yellow Butt Yellow Slant & Red Butt Yellow slant & Green Butt

89. in TSI agar Pseudomonas appears as Red slant & Red butt Black slant & Red Butt Yellow slant & Yellow Butt Yellow Slant & Red Butt Yellow slant & Green Butt

90. MS Agar Stands for Maltose Sucrose agar Selective for S.pyogen Neutral Red is colour indicator S.aureus appear as Yellow colonies S.epidermis appear as Black colonie s

91. EMB agar Stands for Eosin-maltose-Blood agar Selective for G+ve bacilli Neutral Red is colour indicator E.Coli appear as Green sheen colonies S.epidermis appear as Pink colonie s

92. Which of the following is motile bacteria a. All enterobacteriacie except E.coli b. All cocci except S.aureus c. All zoonotics d . pseudomonas e. Mycobacterium

93. Which of the following is non-motile bacteria a. All enterobacteriacie except klebsiella b. All cocci c. All zoonotics except y.pestis d . pseudomonas e. Vibrio cholerae

94. Which is not true regarding Streptococi a. All grow on Blood Agar b. All are catalase – ve c. All are cogulase + ve d. S.agalactiae is CAMP + ve e. All are non motile

95. Which is not true regarding Staphlococi bacteriae a. S.aureus cogulase + ve b. All are catalase + ve c. S.Saprophyticus is Novobiocin + ve d. S.epidermis is Novobiocin + ve e. S.aureus is Mannitol + ve

96. Which is not true about catalase test a. All staphylococci are Catalase + ve b. All enterobacteriacae are catalase + ve c . All Streptococci are catalase - ve d . All Clostridiae are catalase - ve e. All Nisseriae are catalase + ve

97. Which is right about coagulase test a. All staphylococci are Coagulase + ve b . All streptococci are Coagulase + ve c. Yersinia pestis is coagulase + ve d . S.aurus is coogulase + ve e. C & d

98. Which of the following are capnophillic bacteria a. H.pylori & Borreillia b . H.influenzae & Legionella c. H.pylori & Compylobacter d. H.influenzae & N.gonorrhea e. Lactobacillus & S,Pneumoniae

99. Which of the following are Aerotolerant bacteria a. H.pylori & Borreillia b . H.influenzae & Legionella c. H.pylori & Compylobacter d. H.influenzae & N.gonorrhea e. Lactobacillus & S,Pneumoniae

100. Which of the following are Microaerophillic bacteria a. H.pylori & Borreillia b . H.influenzae & Legionella c. H.influenzae & Compylobacter d. H.influenzae & N.gonorrhea e. Lactobacillus & S,Pneumoniae

101. Which of the following is virulence factor of S.pyogen a. Protein A b. C-carbohydrate c. Catalase d. Coagulase e. Exfoliative toxin

102. Which of the following is not virulence factor of S.pyogen a. Protein M b. C-carbohydrate c. Ig Aprotease d. Lipase e. Streptokinase

103. Which of the following is virulence factor of S.aureus a. Protein M b . C Carcbohydrate c. StreptoDnase d. scarlet fever toxin e. TSST

104. Which of the following is not a virulence factor of S.aureus a. Flagella b. Enterotoxin c. Catalase d. Coagulase e. Exfoliative toxin

105. Which of the following is not a virulence factor of N.meningitidis a. Pilli b. Ig A protease c. LOG d. Alpha exotoxin e. Opa Proteins

106. Which of the following is not a virulence factor of N.gonorrheae a. Capsule b. Ig A protease c. LOG d. Pilli e. Opa Proteins

107. Which of the following is not a virulence factor of H.influenzae a. Capsule b. Ig A protease c. LOG d. Pilli e. Lipopolysacharide

108. Which of the following is virulence factor of N.gonorrheae a. Capsule b. Teichoic acid c. LOG d. LPS e. Flagella

109. Which of the following is not similarity between N.gonorrhea & N.meningitidis a. Capsule b. Pilli c. LOG d. Ig A Protease e. Preteins

110. Which of the following is not characterstic of C.tetani a. Terminal Spores ( Drumstick ) b. Spastic Paralysis c. Motile d. Lipase _ ve e. Spreads by wound

111. All are same properties of C.tetani & C.botulinium except a. Anaerobic & non-motile b. Lipase + ve c. Paralysis & exotoxin d. No endotoxin & catalase – ve e. Cultured on cooked meat medium

112. C.tetani & C.botulinium are different in following except a. Paralysis pattern b. Shape of spores c. Lipase test d. C.botulinum spores can also spread by ingestion e. Non-motile

113. C.Perferinges & C. difficle have same characterstics except a. Gram + ve & Spore forming bacilli b. Motile c. Catalase – ve d. Have anaerobic growth e. Sterllization can only be achieved by autoclaving

114. B.Anthracis & B. cereus are having same properties except a. Gram + bacilli b. Spore forming bacteria c . Have Capsule d. Have aerobic growth e. Sterllization can only achieved by autoclaving

115. Regarding Corrynebacterium diptheriae which is true a. Spore forming bacteria b. Diptheria toxin is polypeptide in nature c . Fragment A causes ADP Ribosylation d . Fragment B is toxic part e . Glucose & Maltose – ve

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