Khuram synopsis on bitter gourd lines on heterosis

Bitter gourd ppt

1. HETEROSIS STUDIES IN GYNOECIOUS LINES HYBRIDS OF BITTER GOURD Khuram Danial Shah 19-Arid-4615 M.Sc. (Hons.) Plant Breeding & Genetics Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi. Supervisory Committee: Supervisor: Dr. Rashid Mehmood Rana Co-Supervisor : Miss Ayesha Manzoor Member: Dr. Munir Ahmad Member: Dr. Tanveer Hussain

2. Contents 2 Introduction Literature Review Problem Statement objectives Materials and Methods Expected Outcomes References

3. INTRODUCTION 3

4. Common Name: Bitter gourd Botanical Name: Momordica charantia L. Family: Cucurbitaceae Genus: Momordica Chromosome No. : 2n=22 Origin: Tropical and subtropical areas of Africa & Australia Muronga et al., (2021)

5. Introduction (Kumari et al., 2017) Composition per 100 g Vitamin C (98.5mg) potassium (171mg) Phosphorus (140 mg) magnesium (21 mg) calcium (20mg)

6. Production in Pakistan 6 Area: 4000ha Production:46,000tons

7. Sex expression in cucurbits A wide range of variation in sex forms is present Monoecious, gynoecious, androgynoecious, and gynomonoecious have been observed in cucurbits. 8 Staminate flower Pistillate flower

8. Research Problem 9 The yield and quality of bitter gourd are limited by the lack of understanding of heterosis in gynoecious lines hybrids which hinders the development of high-yielding and disease-resistant hybrids, ultimately affecting the livelihoods of farmers and food security.

9. Objectives:

10. Review of literature Genetic Material Study Parameters Obtained results Reference 7 bitter gourd inbred lines Evaluation of morphological traits Significant differences observed for all the traits Singh et al., (2018) 8 bitter gourd inbred lines Extent of heterosis for quality and yield traits GCA & SCA variance were significant for all traits Mallikarjunrao et al., (2018) Bitter gourd gynoecious lines Utilization in hybrid seed vigor Performed well in commercial hybrid seed production Munshi et al., (2019) Bitter gourd gynoecious lines Maintained by application of chemicals Silver nitrate @ 300ppm performed best A.D. Munshi et al., (2020)

11. MATERIAL & METHODS

12. Experiment 1 13

13. 14 Lines: BG-501 BG-504 BG-507 BG-511 Testers BG-25 BG-77 Aswad Faisalabad Long Safina All crosses will be developed by using Lines x Tester mating design (Kempthorne, 1957). Crossing Scheme

14. Self-pollination in Gynoecious plants Pollens collected from hermaphrodite flower Pollens dusted on stigmatic surface of female flower Developed fruit tagged, containing required information

15. Effect of (AgNO 3 )

16. Experiment 2 17

17. Layout Of Experiment Plant Material: 20 hybrids and 9 parents Design: RCBD Replication : 3 18

18. Data Collection

19. Statistical Analysis

20. Expected Outcome Identification of high yielding and disease-resistant hybrids. Understanding of the genetic basis of heterosis in bitter gourd. 21

21. References  Bakhsh, K., Ahmad, B., Hassan, S., & Gill, Z. A. (2007). An analysis of technical efficiency of growing bitter gourd in Pakistan Punjab.  Pakistan Journal of Agricultural Sciences (Pakistan) .   Behera, T. K. (2005). Heterosis in bittergourd .  Journal of New Seeds ,  6 (2-3), 217-221.   Chaudhary, B. N., Piluek , K., Taychasinpitak , T., & Sagwansupyakorn , C. (2001). Development and maintenance of gynoecious lines of cucumber (Cucumis sativus L.).  Agriculture and Natural Resources ,  35 (3), 242-250.   Dey, S. S., Singh, A. K., Chandel , D., & Behera, T. K. (2006). Genetic diversity of bitter gourd (Momordica 201821-28. Euphytica ,  173 (1), 37-47.   22

22. Dey, S. S., Behera, T. K., Munshi, A. D., & Pal, A. (2010). Gynoecious inbred with better combining ability improves yield and earliness in bitter gourd (Momordica charantia L.).  Euphytica ,  173 (1), 37-47.   Dhillon, N. P., Phethin , S., Sanguansil , S., & McCreight, J. D. (2017). Early staminate flowering monoecious lines have potential as pollenizers for gynoecious hybrid bitter gourd cultivars.  Pakistan Journal of Agricultural Sciences ,  54 (1).   El- Eslamboly , A. A. S. A., & Diab, A. H. M. (2020). New promising squash inbred lines development as a major step to develop superior hybrids.  Egyptian Journal of Plant Breeding ,  24 (3), 555-569.   El- Gazzar , T. M., Tartoura , E. A., & Nada, M. M. (2015). Evaluation of new inbred lines and their hybrids in balady squash variety (Cucurbita pepo L.).  Journal of Plant Production ,  6 (2), 23

23. Thank You

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