KLEBSIELLA

What is Klebsiella? Klebsiella is a Gram-negative rod-shaped bacteria, which belongs to a family of bacteria called the Enterobacteriaceae.
As the channel name suggests, our channel will be a perfect lounge for the malayali medicos..we wil be covering videos which will be like lecture classes related to the subjects biochemistry and microbiology in which we are specialised.. It will be a better learning experience for the students especially for those who are not able to understand and follow the normal classes in college..we assure the students that you will get a basic idea regarding the topic and extra reading can be done from the reference textbooks..

Maneesha M Joseph
MSc MLT (Microbiology)
Assistant Professor
Baby memorial college of allied Health science
Kozhikode
Our Partner Channel
Health & Voyage channel link - https://youtu.be/nzKqRVjlwc0
#Klebsiella
#Medical
#Microbiology
#Biochemistry
#Mallu Medicos Lounge
##MalluMedicosLounge
#MLT
#Channel introduction
#HealthAndVoyage
#New Youtube Channel introduction
#Klebsiella pneumoniae

1. KLEBSIELLA
PREPARED BY,
MANEESHA M JOSEPH
ASSISTANT PROFESSOR
BABY MEMORIAL COLLEGE OF ALLIED MEDICAL SCIENCES
CALICUT
VIDEO CLASS UPLOADED IN YOUTUBE CHANNEL-MALLU MEDICOS LOUNGE

2. || Family =Enterobacteriaceae
| Genus =Klebsiella
| Species =k.pneumonia ,k.ozaenae
,k.rhinoscleromatis.

3. 1.gram-negative
2.Nonmotile
3.Lactosefermenting
4.Oxidasenegative
5.Rod shapedorganism
6.Facultativeanaerobe
7.Surrounded by thickcapsule
8.Act as oppurtunistic humanpathogen

4. 1)Found in the normal flora of the
nose, mouth, skin, GI tract and
intestines.
2)It is also found in soil andwater.
Generally, Klebsiella infectionsare
seen mostly in people with a
weakened immunesystem.

5. 1) urinary tract infections
2)pneumonia
3)Specticaemia
4)nosocomialinfections
5)soft tissueinfections.

6. 1)spread through exposure to the bacteriavia
respiratory tract which causespneumonia.
2)Enters the blood to cause an infection in the
bloodstream.
3)Most well-known in hospitals spread through
person-to-person contact by contaminated hands of
surrounded people in the hospitals, whether it be an
employee or apatient.

7. 4) KLEBSIELLA IS SPREAD VERY EASILY
AND RAPIDLY,BUT NOT THROUGH THEAIR.
5)Healthcare settings are most vulnerable
to Klebsiella infections due to the natureof
procedures that allow easy access of bacteria into
the body. Patients who are on ventilators, catheters,
or surgery wounds are highly prone to catching this
deadlyinfection

9. The symptoms of a K. pneumoniae infection differ
depending on where the infection is located, and
are similar to symptoms of the same diseases
caused by othermicrobes.
For instance, meningitis from K. pneumoniae
produces the hallmark symptoms of bacterial
meningitis, including fever, confusion, neck
stiffness, and sensitivity to brightlights.

10. Bloodstream infections (bacteremia and sepsis)
from Klebsiella cause fever, chills, rash, light-
headedness, and altered mentalstates.
Pneumonia from K. pneumoniae can resultin:
1)Fevers andchills
2)Flu-likesymptoms
3)Cough, which may producemucusthat'syellow,
green, orbloody
4)breathingissues

12. O1)Adhesions
O2)Capsule
OIron Capturingcapability

13. O When the defense mechanism intherespiratoy systemisabnormaleitherdueto illness , age or fatigue our bodylosesfight against microbes then thismicrobeswill multiply rapidly & pass from thetubeinto the alveoli .This will trigger an inflammatory reaction which will generate a lot of infectious fluid that iscomposedfrom microbes , dead antibodies &fluids
from the edges in blood vessels . Then this fluid will block the movement of gases making it difficult to breath. This is mostly caused by klebsiella that invaded the alveoli causing klebseilla pneumonia.

16. Some Klebsiella bacteria have become highly
resistant toantibiotics.
Klebsiella pneumoniae produce an enzyme
known as a carbapenemase (referred to as KPC-
producingorganisms).

17. then the class of antibiotics calledcarbapenems will not
work to kill the bacteria and treat the infection.
Klebsiella species are examples of Enterobacteriaceae,
a normal part of the human gut bacteria, that can
become carbapenem-resistant.

18. •LAB DIAGNOSIS
vThe presence of gram-negative rods in Gram-stained smears
suggestsKlebsiella; they maybe capsulated and non-sporing.
vSAMPLE is inoculated into blood agar and Mac Conkeyagar
medium and incubated aerobically.
GRAM’S STAIN OF KLEBSIELLA

19. vBlood Agar:Mucoid, non-hemolyticcolonies
vMacConkey Agar:Mucoid, lactose-fermenting (pink colored) colonies
vAfter pure cultures have been obtained by selecting colonies from aerobically
incubated plates, they are identified by biochemical reactions and colony
morphology.

24. Indole Production TestNegative (K. oxytocais Indole positive)
Methyl-Red Test Negative
Voges-Proskauer Test Positive
Citrate Utilization TestPositive
Hydrogen Sulfide Production
(TSI) Negative
Urea Hydrolysis TestPositive
Lysine Decarboxylase TestPositive
Arginine Dihydrolase TestNegative
Ornithine decarboxylase testNegative
Motility at 36 °C Non-motile
D-Glucose (acid/gas) Positive/Positive
D-mannitol fermentationPositive
Sucrose fermentationPositive
Lactose fermentationPositive
D-sorbitol fermentationPositive
Cellobiose Positive
Esculin hydrolysis Positive
Acetate Utilization TestPositive
ONPG Test Positive

26. 1)Klebsiella infections that are not drug-resistant can be treated with
antibiotics.
2)Nosocomial isolates ofK. pneumoniaeoften display multidrug-resistance
phenotypes that are commonly caused by the presence of extended-spectrum
β-lactamases or carbapenemases, making it difficult to choose appropriate
antibiotics for treatment.
3)Infections caused by KPC-producing bacteria can be difficult to treat because
fewer antibiotics are effective againstthem.
In such cases, a microbiology laboratory must run tests to determine which
antibiotics will treat theinfection
These drugs include: Aminoglycosides,Polymyxins,Tigecycline,Fosfo
mycin,Temocillin.