Klebsiella oxytoca

slides for your life

1. Klebsiella oxytoca

2. Characteristics Etymology Genus was named after the German bacteriologist Edwin Klebs (1834-1913) Taxonomy Kingdom Phylum Class Order Family Genus Bacteria Proteobacteria Gammaprotebacteria Enterobacteriales Enterobacteriaceae Klebsiella

3. Characteristics Macro Morphology form medium sized, dome shaped, white glistening colonies (2-3 mm in diameter ). Micro Morphology nonmotile cylindrical rod shape bacterium ( 0.3-1.0 x 0.6-6.0 µm), which appear singly, in pairs or in short chains.

4. Characteristics Metabolism Facultative anaerobe Gram- Produce β -lactamase Indole+

5. Epidemiology Habitat Ubiquitous in nature naturally found in the intestinal tract, mouth, nose of humans ; however, they can be found colonizing on all parts of the body . Mode of Transmission P erson to person spread Commonly in healthcare environments

6. Pathogenesis & Spectrum of Diseases Virulence factor Outer membrane composed of lipopolysaccharides (LPS) that have O antigens. LPS layer has a polysaccharide chain known as Lipid A, which produces endotoxins ; capsules ; resistance to multiple antimicrobial agents.

7. Pathogenesis & Spectrum of Diseases Spectrum of Disease & Infection Nosocomial infections of the respiratory tract , urinary tract, blood , and several other normally sterile sites . Most frequently infect hospitalized patients.

8. Laboratory Diagnosis Specimen Collection and Transport Wound, Respiratory tract, Sputum, Nose, Urine (unpreserved) <2hrs Blood Within 2hrs Pleural fluid & IV catheters <15mins Skin &Urine (preserved) Within 24hrs

9. Laboratory Diagnosis Cultivation Organism Medium Appearance Klebsiella spp. MacConkey agar Lactose fermenter; mucoid   Hektoen enteric agar Yellow   Xylose-lysine- deoxycholate agar Yellow

10. Laboratory Diagnosis Identification Lactose fermenter Catalase+ Urease+ Indole+ Methyl red- Gram- Simmons Citrate Agar+

11. Antimicrobial Susceptibility Testing Extended Spectrum β-Lactamase (ESBL) producing organism give effective screening methods for their detection. Helps the doctor give proper antibiotics to patients infected with the multiple antimicrobial resistant Klebsiella oxytoca .

12. Prevention Frequent hand-washing is the best way to prevent spreading the bacteria in your environment. Vaccines