Lab Ware.pptx MLT lab training lab work ppt

Lab work

1. Lab Ware Khursheed Javaid Lecturer Department of Immunology

2. Detail of topics in Clinical, Diagnostic Immunology and Applied Research Lab ware Agglutination Precipitation Electrophoresis , Immuno-electrophoresis and related techniques Western blotting Labelled Immunoassays (ELIA and RIA)   PCR, RNAi, microarray, Quality Control   Production of Chimeric and Hybrid Monoclonal Antibodies Immunization, Vaccines, Methods of vaccine production, Transplantation HLA Typing, MLR Role of the Major Histocompatibility Complex in Mate Choice Hypersensitivity and Allergy Diagnosis   Autoimmunity

3. Lab Items Instruments Chemicals and reagents Lab ware

4. Lab Ware Laboratory glassware refers to a variety of objects used in scientific work Types/Classification: Based on material: Glass/plastic Based on use: To deliver/To contain/To measure Tolerance: Class A – Highest level of accuracy Class B – General purpose, lower level of accuracy

5. Beakers Beakers are used for holding various chemicals. Not for measuring precisely. Sizes vary.

6. Graduated Cylinder Used to precisely measure the volume of liquids or run experiments. Read from the meniscus at eye level. Plastic ring always on top if applicable. Sizes vary.

7. Erlenmeyer Flask Used to approximately measure the volume of various liquids. Useful for mixing by swirling Sizes vary.

8. Florence Flask Used to boil liquids. Also used to collect gases, if applicable. Sizes vary.

9. Volumetric Flask Used to prepare precise standard solutions. They are only good for 1 specific volume. Comes in many sizes

10. Reagent Bottle Used to store, transport, or view reagents such as acids or bases.

11. Dropper and Bottle Used to measure out small amounts of liquids for experiments.

12. Wash Bottle Usually contains deionized water. Handy for rinsing glassware and for dispensing small amounts of dH 2 O for chemical reactions.

13. Goggles and Apron Used to protect your eyes and clothing from damage. These are a must in lab!!

14. Pipet, Pump, and Bulb Used to precisely measure the volume of liquids in small amounts.

15. Berol Pipet Disposable pipets used to transfer small amounts of chemicals. Graduated pipets can precisely measure small amounts of chemicals.

16. Capillary Tubes Used to collect liquid through the process of capillary action.

17. Thank You