Laminar air flow by JIGNESH

Basic laboratory instruments
Principle and working of Laminar Air Flow
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1. Name : JIGNESH.M.PRAJAPATI Roll No : 05 LAMINAR AIR FLOW M.Sc. Sem.- 2 GUIDED BY : Dr. V. A. PATEL SIR

2. LAMINAR AIR FLOW

3. PRINCIPLES OF LAMINAR AIR FLOW Laminar Air Flow provides a work area with Aseptic/sterile conditions for the tissue culture Laminar Air Flow has continuous displacement of air (it provides streamline flow of air) that passes through HEPA (High Efficiency Particulate Air) filter that removes the particulates from the air

4. WHAT IS LAMINAR AIR FLOW? A laminar air flow workstation is a closed cabinet fitted with HEPA filtered air flow system. Here, laminar means unidirectional constant flow of air with almost no or minimal turbulence.

5. The purpose of using such workstations in laboratory is to create particle and bacteria free working environment to carry out specialized work.

6. As these units discharge air towards user, they provide no personal protection but product protection from room contaminants. LAF is short form of laminar air flow .

7. LAMINAR AIR FLOW APPLICATIONS : TYPES OF LAMINAR AIR FLOW 1. Vertical Laminar Air Flow 2. Horizontal Laminar Air Flow Laminar air flow systems are used in various applications such as life science research , microbiology, IVF, plant tissue and cell culture and pharmaceutical and many more.

8. 1. Vertical Laminar Air flow It is the most used type of laminar air flow. In vertical flow cabinet, room air is entered in working area through HEPA filter fitted on top of the cabinet. Thus, air flows downward (vertically) towards work surface and leaves the cabinet sweeping out particles and bacteria

9. 2. Horizontal Laminar Air Flow In horizontal air flow cabinet, room air is entered in working area through HEPA filter fitted on the back side of the cabinet. Filtered air flows horizontally towards the user creating sterile working environment.

10. PARTS OF LAMINAR AIR FLOW CHAMBER Blower : Blower assembly draws room air through pre filter and throws towards HEPA filter

11. Pre Filter : It is primary air filter which filters room air initially and forward towards HEPA filter. Usually, pre filter keeps out particles 5 microns or higher.

12. HEPA Filter This is final filter. Air filtered through pre filter is forwarded towards HEPA filter. HEPA filter offers 99.99% efficiency for 0.3 micron particulates.

13. Pressure gauge Magnehelic gauge is used to monitor differential pressure across HEPA filter with respect to ambient

14. Work bench : It is made of stainless steel. Samples / products are put on it for work.

15. Sash : Sash is sliding front door. User can slide it manually and adjust the opening height as needed. Light : Light is fitted to provide proper illumination under the working area Service fixtures : Main chamber is fitted with electrical socket, air and gas cock.

16. Germicidal UV lamp UV lamp is an added option which is used to sterilize working area prior use. It is advised no contact to made in chamber during this time.

17. Air Filtration : The efficiency of a Laminar Air Flow workstation depends upon the quality of pre filters and HEPA filters. In LAF, the Air filtration system is created to work efficiently even in critical environment. Work Area : The working area is carefully designed for comfortable working and easy cleaning.

18. PRECAUTIONS : Do not the switch on the UV lamp during the working period Do not enter head in the laminar work station or laminar hood Movement of the analyst should be slow or controlled during work to allow Unidirectional air flow Sanitize hands by sanitizing agent to reduce the microbial load.

19. Avoid talking,sneezing ,coughing during microbial work. if any of occurs stop working and follow the cleaning activity and continue the work

20. QUESTION (1) What is full form name of HEPA ? Ans : High Efficiency Particulate Air (2) How many types of Laminar air flow ? Ans : TWO / 1. Vertical LAF & 2. Horizontal LAF (3) Laminar air flow systems are used in various applications such as ………….. Ans :life science research , microbiology, IVF, plant tissue and cell culture and many more.

21. Reference (1) https://www.stericox.com/cleanroom-equipment/laminar-air-flow.html (2) Principles and Techniques of
Biochemistry and
Molecular Biology
Seventh edition
Edited by
KEITH WILSON AND JOHN WALKER