Lec 8 Autoclave. eeeeeeeeeeeeeeeeeeee.pptx

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1. AUTOCLAVE

2. Packing of materials for autoclave There are various types of materials or containers that are suitable for use in an autoclave; all options must have the following properties: be resistant to damage during handling and storage allow the sterilant to penetrate allow the exhaustion of the sterilant after the sterilising cycle prevent penetration of the package by microorganisms after sterilisation

3. Packing of Different types of materials

4. Glassware Glassware such as specimen tubes, petri dishes, specimen collection bottles are wrapped in brown paper and are tied with string Syringes and Needles Syringes are wrapped in gauze and needles are placed separately in small test tubes that are plugged.

5. Cycle Selection Use liquid cycle (slow exhaust) when autoclaving liquids, to prevent contents from boiling over. Select fast exhaust cycle for glassware. Use fast exhaust and dry cycle for wrapped items.