mass production of bio agents and bio pesticides.pptx

presentation on mass production of bio agents and bio pesticides-Agricultural entomology

1. DR PANJABRAO DESHMUKH KRISHI VIDHYAPEETH COLLEGE OF AGRICULTURE ,AKOLA COURSE NO : ELM ENTO 486 COURSE TITLE : MASS PRODUCTION OF BIOAGENTS & BIO-PESTICIDES SUBMITTED TO SUBMITTED BY DR GIRISH JEUGHALE SIR NEHA RAJ T N DR PRERNA B CHIKTE MAM

2. MASS MULTIPLICATION OF CHELONUS BLACKBURNII .

3. INTRODUCTION C . blackburnii   is introduced from Hawaii.  A parthenogenetic egg-larval parasitoid,  C. blackburnii   has a fairly wide host range but in India the common meal moth  Corcyra cephalonica   and potato tuber moth Phthorimaea operculella   have often been used for multiplication of this parasitoid.  It could also be multiplied successfully on  Spodoptera exigua .   C . blackburnii   has been used for the biological suppression of  P. operculella ,  Earias vitella ,  Pectinophora gossypiella   and  Helicoverpa armigera   on cotton and other host plants in many states.  i t is becoming an important component of IPM systems on potato, cotton, etc.,

4. SCIENTIFIC CLASSIFICATION Domain: Eukaryota Kingdom: Animalia Phylum: Arthropoda Class: Insecta Order: Hymenoptera Family: Braconidae Subfamily: Cheloninae Genus: Chelonus Panzer , 1806

5. HOST RICE MOTH ( Corcyra cephalonica ) POTATO TUBER MOTH ( Phthorimea operculella ) TARGETED PEST PINK BOLLWORM SPOTTED BOLLWORM POTATO TUBER

6. MASS MULTIPLICATION MATERIAL Rearing unit of Corcyra cephalonica which includes wooden boxes, crushed grains of sorghum, egg cards, gum Arabic, Corcyra eggs etc. Rearing unit of PTM, which include potato tubers, plastic basket, sterilized soil, puncturing brush, egg sheet of PTM. Breeding glass jars, muslin cloth, rubber bands, scissor, wide mouth plastic jar. Nucleus culture of Chelonus blackburnii adults.

7. METHODS A set of 100, 0-24 hr old eggs of Corcyra (not exposed to UV) are pasted to 5 x 5 cm card. This card containing eggs is exposed to 30 C. blackburnii adults in a 1.5 l container. The plastic container has windows with plastic mesh for aeration. Two cotton swabs, one soaked in 10% honey solution and the other in drinking water are also placed inside from the side opening which is closed tightly with a cloth covered cotton plug. The egg card after exposing to C. blackburnii for 24 hrs is removed and placed on 500 g sterilized cumbu medium. In 30 days time, adults start emerging from the cocoons formed in the cumbu medium after completing development on Corcyra larvae .

8. . The adults live for 25 days and their fecundity is about 400 eggs. The parasitoid could also be reared on potato tuber moth (PTM). A set of 1500 egg of laid on a cloth are stapled to a card. This card containing 0-24 hr old eggs is exposed to 30 C . blackburnii adults. The plastic container (14 cm x 11 cm) is converted into C . blackburnii rearing unit by cutting windows and fixing plastic mesh aeration. Two cotton swabs, one soaked in 50% honey solution and the other in drinking water are also placed inside from the side opening which is closed tightly with a cloth covered cotton plug.

9. . The PTM egg card after exposing to C . blackburnii for 24 hrs is removed and placed on punctured potatoes. This provides more entry points for PTM larvae and kept in a similar plastic container as described for exposure to C . blackburnii . The bottom of this container is lined with sterilized sand. In 25-27 days time, adults start emerging from the cocoons formed in sand at the bottom of the cage or sometimes inside potatoes after completing development on potatoes. The adults live for 23-31 days and their fecundity is about 288-390. Parasitoid host ratio of 1:50 should be maintained and the fresh lot of eggs provided every day

10. FIELD UTILISATION The adult parasitoids could directly be released in the field crops or even in stored potatoes as described for earlier parasitoid DOSES The following release dosages are often adopted, depending on the pest. Potato tuber moth: Two releases @50,000 adults/ release in the field and 5 adults/kg potatoes in godowns . Cotton bollworms: 50,000 adults/ week, first release coinciding with sighting of eggs in the field. Helicoverpa armigera at weekly intervals, first release coinciding with sighting of eggs in the field.

11. THANK YOU .