Mass Production of Fungus and Bacteria and their Quality Control.pptx

Here we r going to look about mass multiplication of fungal biocontrol agents and their quality control

1. Mass Production of Fungus and Bacterial Bio-control agents and their Quality Control

2. Mass Production :- Mass production is the industrial technique to produce large quantities of similar products in constant flows on production lines. Advantages:- Increased Productivity Uniformity Lower cost Faster production

3. Types :- Fungi :- Solid state fermentation Liquid state fermentation Bacteria : - Liquid State fermentation

4. Fungi 1) Solid state fermentation:- Media : solid media Agricultural waste - straw,stubbles,bran

5. Grains - Rice,wheat,sorghum,pearl millet

6. Agricultural by products - Molasses of sugarcane

7. Solid State Fermentation:- Mother culture of Trichoderma Parboiled Sorghum grains (remove the broken,unhealthy grains) Pour the water to the grains Remove the Floating Grains from the water Select the settled grains in the water Dry the grains

8. Pack the grains with the polythene bag Autoclave the grains Inoculate the culture (Trichoderma) on Grains Grind that & Use for the field application

9. Liquid Fermentation Select Mother culture (Trichoderma) Shift the mother culture to the broth (Starter culture) Pour into a Instrument called Fermentor By 3-4 Days Inoculum is produced (T. harzianum - potato dextrose broth, molasses-yeast medium, and V-8 juice)

10. Fermentor:- A device used for the sterilisation and enclosed vessels that are used for the growth of microorganisms under optimal conditions

11. 1. Carrier Based Formulations - Widely used carrier material is talc CMC(Carboxyl Methyl Cellulase) + Talc + Broth culture 2. liquid Based Formulations - Solutions,Suspention,EC etc..

12. Bacteria King’s B medium (broth) is ideal for mass production of antagonistic bacteria, however other media like Tryptic Soya Broth or Nutrient Broth can also be used.

13. Quality Control :- Fungus :- Identification and characterization Viability and Germination Pathogenicity and Efficacy Contamination

14. Bacteria Identification and Characterization Viability and Cell count Pathogenicity and Efficacy Contamination

15. THANK YOU