Mass production procedure for Neoseiulus longispinosus

The given ppt show that the mass production procedure for the predatory mite,which also include their
taxonomic information and working principles in biological control and given data is from some research paper and books.

1. WELCOME

2. Name of Student : Yugant Manoj Patil Reg .No.: AS-2020/120 Course No : SRP –EL-ENTO -406 Course Titile : Mass Production of Bioagent and Biopesticide Topic name : Mass Production of Neoseiulus longispinosus DEPARTMENT OF AGRIL. ENTOMOLOGY

3. Neoseiulus longispinosus . Kingdom- Animalia Phylum -Arthropoda Class -Arachnida Subclass - Acari Order - Meostigmata Family - Phytoseiidae Genus - Neoseiulus Species - longispinosus TAXONOMY

4. INTODUCTION Neoseiulus longispinosus mites are widely used to control spider mites, and are commercially available. This has to be multiplied on spider mite ,which turn have to be raised on suitable plant. They give cannibalism a good name, as they eat related spider mites by the dozens every day.

5. BIOLOGY Neoseiulusl ongispinosus is soft-bodied and translucent pale brown to sometimes tan-colored depending on the food consumed. Adult mite is pear shaped and may range between 0.5-1.0 mm in length. Due to its small size, it is not easily visible with the naked eye and can be best seen with a hand lens of at least 10× magnification or under a stereoscope. On plants in the field and greenhouse

6. Life cycle Stage Period Egg 3 days Larve 2 days Nymph 7 -10 days Adult 28-35 days However, these biological parameters change depending on available food sources

7. Egg : Egg is oval, translucent white .An adult female produces an average of 35 eggs during her lifetime. Eggs are laid on the leaf surface, on domatia or on hairs along the midrib on the underside of leaves, and occasionally on petiole hairs. Larvae: Larvae are tiny, oval, translucent shiny white to tan, with six legs; wingless

8. Nymph: The two nymphal stages ( protonymph and deutonymph) last for seven to 10 days before developing into adults Adult: Pear-shaped; shiny translucent white but turning pale tan, orangey, reddish or green after feeding; eight legs; wingless.

9. Mass Multiplication of Neoseiulus longispinosus Prepare pot mixture of red soil and FYM ↓ Sterilized soil by mixing formaldehyde 5% and covering the mixture with a polythene sheet ↓ Add Trichoderma hazarium culture ,@ 1 kg/ton of pot mixture to prevent pythium ↓ Fill 14”X 12” pots earthern with pot mixture ↓ Procedure

10. ↓ Sow french bean seeds 4 seeds per pot ↓ Transfer the pots to the polyhouse ↓ Water the pots regularly,add 3g/pot DAP and 2g /pot MOP -15 DAS ↓ 20 DAS the French bean seed release the spider mite Tetranychus urticae at four mite per leaflet ↓

11. ↓ Ten days after spider mite have been released,release the phytoseiid mite at four mite per leaflet ↓ Maintain the pots by watering , wedind ,thinning when required. Take care that pesticide/ rain do not affect the spider mite ↓ Water the pots regularly,add 3g /pot DAP AND 2g /pot ↓ Maintain the plant such that predator can develop without being affected by pesticide , excess water /temperature . ↓

12. ↓ 23 days after release of predator ,the leaves of French bean plant can be clipped and transfer to ployhouse where biological control of spider mite has been planned HOST SPECIES : Tetranychus urticae (Red spider mite and two spotted spider mite), Panonychus citri ( Citrus red Spider mite) Spider mites (family Tetranychidae) primarily, but also mites in the families Tarsonemidae and Tydeidae , thrips, and other small arthropods

13. THANK YOU ! …