Mechanism of action of lysozyme

It represents the enzyme lysozyme with its structure and its mechanism of action on the components of the cell layers.

1. Mechanism of Action Of Lysozyme Akshay Wakte

2. Introduction Lysozyme is also called as muramidase or N- acetylmuramide glycohydrolase . It is antimicrobial enzyme and small globular protein, and present in tears, nasal mucous, gastric secretion, milk and egg white. It lyse the bacterial cell wall. Turn over no. : 0.5/sec. and E.C. no is 3.2.1.17 In 1922, Alexander Fleming coined the term lysozyme.

3. Structure of Lysozyme In 1965, David Phillips and his colleague’s determined 3D structure by X-ray crystallography. Molecular weight : 14.3 Kda Shape is ellipsoidal and dimension are 45X30X30 A o It contain polypeptide chain of 129 amino acid Lysin at the N terminal and Leucine at the C terminal.

4. Structure of Lysozyme Active site of lysozyme are of six subunit which are bind to six substrate molecules.

5. MECHANISM OF ACTION OF LYSOZYME Glutamate-35 and Aspartate-52 are present at active site. After binding, D residue is distorted half-chair conformation. Glu-35 transfer its proton to oxygen and break the bond and generate carbonium ion . Asp-52 s tablize that ion , E ring will released. Followed by addition of water, enzyme will regenerated and ring D will be released.

6. Effect of pH on mechanism of Lysozyme

7. Thank you!