MF Membrane filtration techniques for coliform bacteria

This presentation will cover the membrane filtration techniques for coliform bacteria detection we will discuss principal procedure and quality control

1. MF Technique for Coliform Bacteria This presentation will cover the Membrane Filtration (MF) technique for coliform bacteria detection. We will discuss principles, procedures, and quality control. Let's explore the advantages and limitations of this essential method. Presented by : Meghana Puranik BSc SEM VI Dept of BIOTECHNOLOGY Hislop College , Nagpur

2. What are Coliform Bacteria And Why are they Important? What are Coliforms? Coliform bacteria are indicators of water quality. They are commonly found in the environment. Why are they Important? Their presence suggests potential fecal contamination. Coliform bacteria can cause diseases such as cholera.

3. 3 Principles Behind the Membrane Filtration (MF) Technique Filtration Water sample is filtered through a membrane with small pores. Incubation Membrane is placed on selective media and incubated. Colony Growth Coliform colonies grow on the membrane surface. Quantification Colonies are counted to determine coliform concentration.

4. 4 Required Materials and Equipment for MF Technique Membrane Filters Sterile filters with a specific pore size. Filtration Unit A device to hold the filter and pass water through . Selective Media Nutrient agar that encourages coliform growth . Incubator Maintains optimal temperature for bacterial growth .

5. 5 Incubate at correct temperature. Count Colonies Count colonies after incubation. Incubate Step-by-Step Procedure for MF Technique Prepare Sample Mix and dilute water sample . Filter Pass sample through membrane filter. Place on Media Put the membrane on selective agar. 1 2 3 4 5

6. 6 Interpreting Results: Colony Counting and Identification 1 Counting Colonies Count all typical coliform colonies. 2 Calculating CFU Calculate colony forming units (CFU) per volume. 3 Identification Confirm coliforms with biochemical tests .

7. 7 Quality Control and Assurance in MF Technique Sterility Ensure sterile materials and equipment. Controls Use positive and negative controls. Perform replicate analysis to assess variability. Replicates

8. 8 Advantages and Limitations of MF Technique Advantages Easy to perform Cost-effective Limitations Turbid samples can clog filters Overgrowth can occur Requires skilled technicians High sample volume

9. Thank You Acknowledgement Sincere gratitude to Department of BIOTECHNOLOGY for their unending support and guidance.