Micropropagation

micropropagation, definition, procedure, advantage, disadvantage and application

1. Presentation on Micro-propagation By :-Manoj Kumar Saini Department of Genetics and Plant Breeding RLBCAU, Jhansi 3 November 2019

2. Introduction Micro-propagation is the p roduction of whole plant from small selection of plant such as a stem tip, node, meristem, embryo, or even a seed. It is the only commercially viable method of clonal propagation of most of the horticultural crops. E.g. Orchids

3. PROCEDURE

4. Advantages of micro-propagation:- Quick mass multiplication Off season plant production Production of morphological similar plants Disease free plants Useful for those species that are difficult to grow through seed Breeding cycle can be shortened In vitro culture can be stored for long time through cryopreservation

5. Disadvantages of Micro-propagation:- Very expensive and have high labour cost An infected plant produce infected progeny Not all plants can be successfully tissue cultured Plants are not autotrophic More problem in inducing root

6. Application of Micro-propagation :- Somaclonal variation Germplasm conservation Mutation breeding Inducing breeding Embryo culture Haploid and diploid production Genetic engineering Production of secondary metabolites Production of disease free plants In vitro Hybridization –protoplast fusion

7. Thank You