Microtome knife profile

A Presentation file In PDF format, Describes Microtome Knife profile

1. Faris K
Department of MLT
MCH Trivandrum

2. KNIFE PROFILEKNIFE PROFILE
Knives are modelled according to the tissue to be
sectioned and the embedding medium.

3. KNIFE PROFILESKNIFE PROFILES
Many knife profiles

profile A, B, C & D (Histotechnology)

4. PROFILE A (PLANO CONCAVE)PROFILE A (PLANO CONCAVE)
One side of cutting surface is flat and the other is
concave with different degrees of concavity
Extremely sharp, but delicate
Used for cutting soft samples like nitrocellulose
embedded tissues
The plane surface is closest to tissue block
Sledge and rotary microtome

5. PLANO CONCAVEPLANO CONCAVE

6. PROFILE B (BICONCAVE)PROFILE B (BICONCAVE)
It is the classical knife shape with concavity on
both sides
Introduced by Heiffor
Rocking microtome and sledge microtome
Length 100-250mm
Less rigid
Prone to vibration

7. BICONCAVEBICONCAVE

8. PROFILE C (WEDGE)PROFILE C (WEDGE)
Use in all type of microtome to cut all type of
materials
Both cutting surface are plane
Known as standard profile
Size 100-350mm

9. WEDGE KNIFEWEDGE KNIFE

10. PROFILE D (TOOL EDGE)PROFILE D (TOOL EDGE)
Wedge knife with steep cutting edge
Hard object( un-decalcified bone)
Stouter to give rigidity
Tungsten carbide coated edges(rigidity)
Have detachable handle (to screw with holder)

11. CONTI..….CONTI..….
Wedge knife is stable for long time
Need more force to achieve the cut
Varying length available
80mm --freezing microtome
240mm-- base sledge microtome