Molecular Motors, its introduction, types, basic components, animation to understand, regulation and study processes

Molecular Motors, its introduction, types, basic components, animation to understand, regulation and study processes

1. Molecular Biology Molecular Motors

2. Defining Molecular Motors

3. The CHEMO-MECHANICAL Cycle Chemical energy (ATP hydrolysis) to Mechanical Energy (non-random motion)

4. Role of Molecular Motors Help move Organelles, proteins, vesicles, and mitochondria (cargo) from the nucleus to the periphery and vice versa. Molecular machines transform energy into useful work. They play a role in cell division by pulling the chromosomes and transporting synaptic vesicles including neurotransmitters from the afferent neuron body to the synaptic terminal. Molecular motors are based on protein and perform intracellular movements responding to stimuli.

5. Key Components of Molecular Motors

6. Transcriptional motors 1- Kinesin 2- Myosin 3- Dynein Rotatory Motors Helical flagella in E.coli Polymerization Motors 1- Actins 2- Microtubules Molecular Motors Types Based on Action

7. Types Based on Path Followed by Transcriptional Motors

8. Example of Molecular Motors with Animation Sr No. Example Types Action 1 Cytoskeletal Motors Myosin 1- Muscle Contraction 2- Intracellular transport 3- Production of cellular tension (moves toward +ve end of microtubules) Kinesin 1- Inter-cellular transport (nucleus to cell) (Anterograde Transport) (Moves towards + ve End of the actin) Types: 1 (conventional),2 (heterometric),5 (bipolar),14 Dynein 1- Axonemal beating of cilia and flagella 2- Inter-cellular Transport (cell to nucleus (Retrograde Transport) Moves toward – Ve end of the Microtublues = Power Stoke means crawling)

9. Example of Molecular Motors Sr no. Example Action 2 RNA Polymerase (enzyme) Transcribe RNA to DNA DNA Polymerase (enzyme) Transcribe single stand DNA to Double stand DNA Topoisomerase Reduce DNA’s Supercoiling Helicase Separate Double-stand DNA before Transcription or replication.

10. Animation of Molecular Motors

11. Techniques to study Molecular Motors

12. Regulation of Molecular Motors

13. Any Questions