morphology and types of cells in culture lecture 3

Based on shape and appearance: 3 types
Fibroblast-like
Epithelial-like
Lymphoblast-like

1. Morphology and types of Cells in Culture LECTURE OF SUBJECT: Dr. sharafaldin Al- musawi College of Biotecholgy LECTURE : 3 SUBJECT : Animal Tissue culture LEVEL : 4

2. Fibroblast-like Epithelial-like Lymphoblast-like Based on shape and appearance : 3 types

3. Fibroblast-like cells are bipolar or multipolar, have elongated shapes, and grow attached to a substrate Morphology of Cells in Culture

4. Epithelial-like cells are polygonal in shape with more regular dimensions, and grow attached to a substrate in discrete patches. Morphology of Cells in Culture

5. Lymphoblast-like cells are spherical in shape and usually grown in suspension without attaching to a surface. Morphology of Cells in Culture

6. Anchorage-dependent Anchorage-independent Morphology of Cells in Culture Depending on the adherence property : 2 types

7. Anchorage-dependent ( adherent or monolayer culture ): Must be cultured while attached to a solid or semi-solid substrate Example: MCF7 , PC-3 Anchorage-Independent ( suspension culture ): Can be grown floating in the culture medium Example: K562 Morphology of Cells in Culture