Optical microscope

Types, Components, Principle, Application, Limitations and New discovery in field of Microscopy

1. A PRESENTATION ON OPTICAL MICROSCOPE M.L.V. GOVT. TEXTILE AND ENG.COLLEGE By:- SAURABH SHANKAR

2. CONTENTS 1. INTRODUCTION 2. FIRST BREAKTHROUGH 3. VIEWING HEADS 4. TYPES 5. COMPONENTS 6. WORKING PRINCIPLE 7. BASIC FUNCTION 8. MAGNIFICATION 9. APPLICATIONS 10. LIMITATIONS 11. 3-D MICROSCOPY

3. Optical Microscope is a type of microscope which uses visible light and a system of lenses to magnify images of small samples. The image from an optical microscope can be captured by normal light-sensitive cameras to generate a micrograph. WHAT IT IS ?

4. Dutch spectacle makers, Zacharias Jansen and his father Hans were responsible for making the first compound microscope in the late 16th century FIRST BREAKTHROUGH First compound microscope

5. Monocular - only use one eyepiece when viewing the specimen. I nexpensive Binocular - with two eyepieces which proves to be more comfortable. M ost common choice. Trinocular - has a third eyepiece tube that can be used by another person simultaneously or by a CCD camera. Most expensive . VIEWING HEADS

6. TYPES Upright Inverted Research Metallurgical Measuring

7. COMPONENTS Eyepiece Objective turret Objective lenses Stage Diaphragm and condenser Light source Mechanical stage Coarse adjustment Fine adjustment

8. WORKING PRINCIPLE

9. BASIC FUNCTION

10. MAGNIFICATION Magnification of a compound optical microscope is the product of the powers of the ocular (eyepiece) and the objective lens. I f the magnification of the eyepiece is 10x and the magnification of the objective lens is 40x , the overall magnification is 400x. The maximum normal magnifications of the ocular and objective are 10× and 100× respectively, giving a final magnification of 1,000×.

11. M icroelectronics , nanophysics, biotechnology, pharmaceutical research, mineralogy and microbiology M edical diagnosis, the field being termed histopathology when dealing with tissues, or in smear tests on free cells or tissue fragments. Measuring microscopes are used for precision measurement. APPLICATIONS In I ndustrial use, binocular microscopes are common.

12. LIMITATIONS At very high magnifications with transmitted light, point objects are seen as fuzzy discs surrounded by diffraction rings , airy disks. I mpacts of diffraction limit the ability to resolve fine details. Airy Disks

13. 3-D OPTICAL MICROSCOPY 3-D microscopy images (upper) and SEM images (lower) of Si samples, laser treated at 25 kHz (A), 70 kHz (B) and 100 kHz (C )  

14. THANK YOU