Organoid 3 d materials. New tiquenice for

3 d organoid

1. 3–D organoid Muhammed faizal a a

2. About organoid Professor Hans Clever introduced first organoid from stem cells. Organoids are generated from stem cells with Presence of growth factors and matrix Normally provided by Stem cell In body. Organoids conserve parent stem cell mutation Characteristics and parent stem cell gene expressions. They will maintain the parent cell biological and functional characteristics for long time.

3. Why organoid?.. They are very good for New drug discovery Drug screening Effect of medicine in various tisues Disease modelling Vulnerability of infectious agent in various tissues

4. Limitations of organoids Lack of vascular system :- In normal body tissues the blood constantly supply nutrients and exchange oxygen and waste. Organoids lack blood vessels they growing in nutrient rich medium but as they grow the inner cells will not get nutrients. As today organoids are not yet developed to integrate immune cells and tumour cells. Tumor cell with an established connection with immune cell will help to understand more and help in more relevant studies

5. Zika and microcephaly In 2015 there was an spike of cases of infants with microcephaly . And the mother was infected with Zika virus. Microcephaly:- a medical condition that infants born with abnormally small head and brain. And they have chronic developmental and neurological issues. They need life long medical attention. Zika virua .:- discovered 80 gears ago in zika forest but not yet publicised at that time. At till this time no report of causing developmental or neurological issues in individuals As the cases were increasing no one could understand is the zika causing microcephaly..

6. WHO DECLARED INTERNATIONAL EMERGENCY As no one could understand tha relation btw Zika and microcephaly. People were feared of it. The reson that no reasearchers could not do anything is the inaccesbility of delveloping nervous system for controlled studies. At that time prof guo -li- ming genrated a ‘brain organoid’

7. PROJECT ZIKA & ORGANOID As they have organoid the cantacted her friend who work as virologist. They joined together and started the project. As theoretically it easy but in practical it was very hard because they have to build different medium and spinning bioreactor.

8. First week and lster results