Partition column-chromatography

THE pptx file on partition column chromatography

1. 1 Welcome

2. Presentation on : P artition C olumn C hromatography Course code : ACCE3105 Course Title : Instrumental Method of analysis- I Submitted to: Rajib Chandra Das Lecturer Dept. of ACCE,NSTU NOAKHALI SCIENCE & TECHNOLOGY UNIVERSITY Department of Applied Chemistry & Chemical Engineering Date of Submission: 11/07/18 2 Presented by ASIF PAPPU

3. Partition C olumn C hromatography Chromatography Partition chromatography Types of partition chromatography Principle of partition column chromatography Application of partition column chromatography 3

4. Partition Chromatography • Method of separation in which the components present in the mixture get distributed more likely into two liquid phases because of differences in partition coefficients during the flow of mobile phase in the chromatography column. 4

5. Partition Chromatography The most widely used type of HPLC is partition chromatography . The stationary phase is a second liquid that is immiscible with the liquid mobile phase . Earlyr in PC used liquid liquid column but in modern LC systems liquid bonded phase column is used. In liquid liquid chromatography the liquid was held in place by physical adsorption but in liquid bonded phase column system, attached by chemical bonding resulting in highly stable packing insoluble in the mobile phase. Bonded - phase columns are also compatible with gradient elution technique 5

6. Column for Bonded-Phase Chromatography 6 In maximum bonded phase packing for partition chromatography are prepared from rigid silica or silica based compositions .This is uniform porous and mechanically study . Gradient elution – Mobile phase content changes during elution Isocratic elution – Mobile phase is all the time the same during elution

7. 7 Difference Between Adsorption and Partition Chromatography • Partition chromatography - separation on the stationary phase occurs by partition due to differences in partition coefficients. • Used for liquid-liquid or liquid-gas chromatography • Adsorption Chromatography -relative differences in adsorption of constituents of given sample. Because of differences in their affinity towards stationary phase, the components of the mixture adsorb with different rates . • used only for solid-liquid or solid-gas chromatography .

8. Partition Chromatography Principle • Separation of components of given sample occurs due to partition of components between two liquid phases. • Stationary phase is coated with a liquid which is immiscible in mobile phase. • Stationary phase immobilizes the liquid surface and makes it stationary phase. • The mobile phase passes over the stationary phase and separate out. • The separation depends on the relative solubility in the stationary liquid layer because of different partition coefficient, different component of sample are separated. 8

9. Partition chromatography 9

10. Types of Partition Chromatography 10 Partition Chromatography Liquid-Liquid C hromatography Gas-Liquid Chromatography

11. Liquid - Liquid Chromatography Partition or liquid-liquid chromatography ( LLC): A powerful separation technique which is used for the separation and analysis of acids and proteins. The basis of LLC is the distribution of sample molecules between two immiscible liquid phases, a stationary phase and a mobile phase (Figure 1 ). In conventional LLC , the stationary phase is mechanically held to a support by adsorption . E mploys liquid mobile and stationary phases. U ses small particles with molecules bonded to their surface to give a thin film that has liquid like properties. 11

12. Liquid-Liquid Chromatography(LLC) 12

13. A pplication of LLC Separation of compounds containing metals. Determination of compounds in biological samples. Analysis of drugs in food products. Determination of compounds in environmental samples. Determination of compounds in biological samples. Separation of important organic chemical. 13

14. Partition Chromatography Applications • used for final purification natural extracts, synthetic mixtures and biological matrices. • It is also used for fractionization of complex crude extracts eg . Petroleum fractions • Determination of water quality. • Separation of aroma molecules of wine. • Determination of pesticide residue. 14

15. References http ://www.waters.com/webassets/cms/category/media/other_images/primer_T_Ion_Exchange _ CHromatography.jpge http://xray.bmc.uu.se/Courses/MPC/students_files/ION_EXCHANGE_CHROMATOGRAPH Y.pptx http :// www.thefreedictionary.com/chromatography http :// www.britannica.com/EBchecked/topic/115917/chromatography/80518/Liquidchromatography http :// www.ltt.com.au/simulab/5/Laboratory/StudyNotes/snClassifChromatoMeth.html 15

16. The End! Thank you ! 16