Pentoser Phospahte Pathway

The pentose phosphate pathway is required to produce the 5 ribonucleotides, the NADPH for free radical excretion, and ribonucleotide for synthesizing the nucleosides and nucleotides.

1. Biochemistry Seminar Pentose Phosphate Pathway M sc;biotechnolgy

2. CONTENT; INTRODUCTION & HISTORY OVERVIEW REACTION ENZYMES ACTION SIGNIFICANCE REFERENCE

3. INTODUCTION

4. HISTORY OF PENTOSE PHOSPHATE PATHWAY Sir Hans Adolf Krebs and Fritz Albert Lipmann

5. OVERVIEW Totally 10% if the glucose enters into pentose phosphate pathway Out of that 10% - (30%) of the pathway happens at the liver. i.e 100g 10g 3g This particular reaction takes place in the cytosol and particularly important in the tissues like adipose tissue,mammary gland & adrenal cortex The PPP is very low in the skeletal muscle The core set of this pathway is to oxidize glucose 6-phosphate to ribose 5-phosphate and generates NADPH as well as converting hexose into pentose The main product of this patheway is NADP and the ribose 5-phosphate.

6. REACTION Glucose 6-phosphate + 2NADP+ +H2O ribose 5- phosphate + 2NADPH +2H+ +CO2

7. STAGES There are three stages involved in this pathway Stage-1; (Oxidative reaction) Stage-2; Isomerization of ribulose 5-phosphate to ribose 5 – phosphate Stage-3; linking of the pentose phosphate pathway with the glycolysis Via transketolase & transaldolase (Non oxidative reaction)

8. STAGE-1(oxidative phase) H -

9. STAGE-2 (Isomerization phase) NON OXIDATIVE PHASE

10. STAGE-3

11. ENZYME ACTION

12. SIGNIFICANCE

13. REFERENCE