Peptic ulcertherequiredtosubmitPresentation1.pptx

Peptic ulcer

1. Anti - Ulcer effect of Asl-us- Soos on Mefenamic acid induced peptic ulcer in rodent model

2. 8. References 7. Study design 5. Material
and method 6.
Methodology 4. Objective
of the study 3. Research
design 2. Review
of literature 1.Need of the
study CONTENTS

3. NEED OF THE STUDY Peptic ulcer is a sore or erosion in the lining of the digestive tract, typically in the stomach or small intestine. This disease ranks among the prevalent gastrointestinal conditions, contributing significantly to morbidity rates. Almost 15,000 people lose their lives each year as a consequence of Peptic Ulcer. The life time risk for developing a peptic ulcer is approximately 5% to 10% with the rate of 0.1% to 0.3% per year . According to the latest WHO data published in 2020 Peptic Ulcer Disease Deaths in India reached 68,108 or 0.80% of total deaths.

4. In modern medicine, proton pump inhibitors, anticholinergics, antacids, antimicrobial agents, H2-receptor antagonists, sucralfate, and bismuth are used for cure of peptic ulcer but these are not entirely efficient and also can cause various adverse effects such as impotency, arrhythmia, haemopoietic alterations , hypersensitivity reactions, and gynecomastia Herbal medicines proven effective for treatment of peptic ulcer with no side effect, hence most of the world population prefer it over modern medicine.

5. In Unani classical literature there are various drugs which are in used from ancient period for Qarah -e Medi , like- Elva ( Aloe barbadensis Mill), Isapghol ( Plantago ovata) , Asl-us- soos ( Glycyrrhiza glabra Linn ), Habbul Aas ( Myrtus communis linn . ), Kundur ( Boswellia serrata Roxb .) Amla ( Emblica officinalis Gaertn ), Anjabar ( Polygonum bistorta L. , Jaiphal (Myristica fragrans Houtt .) Rasaut ( Berberis vulgaris L .), Khurfa ( Portulaca oleraceae Linn ), Rewand chini ( Rheum emodi wall.ex Meissn ) My experimental drug is Asl-us- soos which has already been experimentally tested for the anti-ulcer effect. In this study we will induce ulcer through the drug Mefenamic acid in animal model which still has not been used for this study with Asl-us- soos .

6. REVIEW OF LITERATURE In Unani Medicine, peptic ulcer is known as Qarah –e- Meda . Unani scholars mentioned its causes as Khilte Haad (hot and irritant humour ), Fuzlat (waste products), Intake of hot and spicy foods, Irregular dietary habit, E xcessive intake of rotten food, A lcohol and hard fibrous diet, Desensitization of internal surface of stomach which causes excessive gastric secretions, C hronic gastritis and indigestion, P rolonged stress and strains U nabsorbed gastric secretions.

7. DESCRIPTION Asl-us- soos consist of dried unpeeled roots & stolons 14-20 cm in length & 2 cm in diameter . Botanical name: Glycorrhyza glabra Family: Fabaceae Distribution: Commercially cultivated in Spain,Sicily&England Habit- herbaceous perennial shrub growing to 1 m in height, Flowers- 1 cm long, light violet in colour , Fruit- 1–3 cm long , an oblong pod Leaves- odd multifoliate pinnate leaves in 4-7 pairs . The roots are harvested 3-4 years after planting .

8. ACTION Munzij -e- akhlat -e- ghaliza ( concoctus of viscous humour ), musakkin (sedative), gasil azae batni (abluent of internal organs), muqawwi (tonic), kasir -e- riyah (carminative), mulattif , munzij -e- balgham (concoctive of phlegm), jali (detergent), mulayyin (laxative) mudir bol wa haiz (diuretic & emmenagogue) , musakkin atash (thirst allaying), muqawwi aasab (nervine tonic), munaffis -e balgham (expectorant), dafe tap-e- muzmena (antipyretic for chronic fever) dafe suzish meda (anti gastritis), maullid mani (semen productive) mushtahi (appetizer), muhallil -e- riyah (flatulence dissolvent) mundamile qarooh (wound healer).

9. USES As per Unani physicians Asl-us- soos gives strength to nerves, beneficial in cough, sore throat, hoarseness of voice, burning micturition, gonorrhea, qurooh -e- masaana wa gurda . It is also used in some diseases of liver and spleen. It relieves nervine pain, asthma, lung diseases. Its decoction used to expel out balghami ratoobat from stomach. Therefore, helpful in flatulence, gastritis, piles, chronic fever, and thirst. It is beneficial in diseases of brain and nerves and useful in palpitation. It is used in nausea, vomiting, and fasaad -e- safra . Its decoction is used to improve vision power. It is useful to cure headache and migraine.

10. TEMPERAMENT Hot in 2 nd degree and Dry in 1 st degree. HUMAN DOSE-----6-9gm

11. OBJECTIVE OF THE STUDY To evaluate the antiulcer effect of Asl-us- soos in mefenamic acid induced peptic ulcer To verify the assertion made by unani scholars

12. RESEARCH DESIGN Antiulcer activity

13. Material and method Collection of Drug : The experimental plant Asl-us- soos , will be procured from the herbal garden of Govt. Tibbi College and Hospital, Patna or will be purchased from local herbal drug store-Patna and will be identified by the Pharmacognosist . Preparation of Extract: The roots of Asl-us- soos , will be collected, dried in shade and grinded into coarse powder. 10 g of this powder will be mixed with 250 ml of water and the aqueous extract will be prepared using Soxhlet’s apparatus.

14. EXPERIMENTAL ANIMAL Wister Albino Rats of weight around 150-200 grams each of either sex will be randomly selected for this study. All the animals will be purchased from Laboratory Animal Facility, Patna, Bihar, India. Two to three months old Albino Wistar rats (150-200 g) of either sex will be used in all the tests. Before each experiment, the animals will be acclimatized for a week and housed in clean polypropylene cages. Each animal will be kept in separate cage to rule out possibility of estrous synchrony. The animals will be kept at room temperature of 25 ℃, 45-55% humidity, 12-hour light and dark cycle, standard diet and water ad libitum . Experiment protocols and procedures of animal care are in accordance with CPCSEA guidelines

15. Methodology: Induction of Peptic Ulcer Peptic ulcer will be induced by single dose of mefenamic acid suspended into 1% carboxymethyl cellulose orally 1200 mg/kg body weight in healthy rats.

16. Study Design The experimental animals will be divided into 8 groups of 6 animals each in the manner below: Group 1: Plain control group will be administered with distilled water by gavage. Group 2: Negative control group will be administered with mefenamic acid suspended into 1% carboxymethyl cellulose by gavage 1200 mg/kg body weight once daily for five days. Group 3: Standard control Pre-test group will be administered with Omeprazole 20 mg/kg once daily for five days and mefenamic acid 1200 mg/kg for next five days. Group 4: Test control Pre-test group A will be treated with single dose of aqueous extract of roots of Asl-us- soos (0.7 mg/kg) once daily for five days and mefenamic acid 1200 mg/kg for next five days. Group 5: Test control Pre-test group B will be treated with double dose of aqueous extract of roots of Asl-us- soos (1.4mg/kg) once daily for five days and mefenamic acid 1200mg/kg for next five days. .

17. CONT. Group 6: In Standard control Post treated group ulcer will be induced with mefenamic acid 1200mg/kg for five days and then treated with Omeperazole 20mg/kg once daily for next five days. Group 7 : In Test control Post treated group, A ulcer will be induced with mefenamic acid 1200 mg/kg for five days and then treated with single dose of aqueous extract of roots of Asl-us- soos ( 0.7mg/kg) once daily for next five days. Group 8: In Test control Post treated test group B ulcer will be induced with mefenamic acid 1200 mg/kg for five days and then treated with double dose of aqueous extract of roots of Asl-us- soos (1.4mg/kg) once daily for next five days. In pretreated group after 5 days of treatment (after 24 hour of fasting) on 6 th day ulcer will be induced by toxic drug mefenamic acid dissolved in CMC for five days then animals will be sacrificed and their stomach will remove to examine extent of mucosal damage in terms of ulcer index. In post treated standard and test group first ulcer will be induced by toxic drug mefenamic acid dissolved in CMC for five days then treatment will be continued for five days in same manner. Then animals will be sacrificed and their stomach will remove to examine extent of mucosal damage in terms of ulcer index

18. STATICAL ANALYSIS All the values will be expressed as +_ SEM. The statistical significance will be determined by one-way ANOVA test. Values P <0.05and P <0.01 will be considered as significant. And P <0.001 as highly significant.

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