Phospholipase C IP3 DAG Pathway

Mechanism of how Gq type G Protein coupled receptor works, Signal transduction mechanism of Gq GPCR

1. Presented by- Mukul Sunil Tambe. M. Pharm Sem.I Pharmacology. PHOSPHOLIPASE C IP3 DAG PATHWAY 10/22/2016 1

2. PLC is a class of membrane-associated enzymes that cleave phospholipids just before the phosphate group. It has important role in Signal Transduction. Binds to G α q subunit of G- Protein Coupled Receptor and activates it. Activators - Heterotrimeric G protein subunits, Protein Tyrosine kinases , Small G proteins, Ca 2+ and Phospholipids. PHOSPHOLIPASE C (PLC): 10/22/2016 2

3. ISOTYPE TYPES Beta ( β ) PLCB1, PLCB2, PLCB3, PLCB4 Gamma ( γ ) PLCG1, PLCG2 Delta ( δ ) PLCD1, PLCD3, PLCD4 Epsilon ( ε ) PLCE1 Eta( ζ ) PLCH1, PLCH2 Zeta ( η ) PLCZ1 Types of Phospholipase C: 10/22/2016 3

4. IP3 and DAG: Phosphatidylinositol 4,5-bisphosphate Inositol 1,4,5-trisphosphate Diacyl glycerol 10/22/2016 4

5. Mechanism Of Enzyme: 10/22/2016 5

6. https://en.wikipedia.org/wiki/Phospholipase_C. https://www.youtube.com/watch?v=2bbBrpgeheY. https://www.google.co.in/search?q=phospholipase+c&biw=958&bih=548&source=lnms&tbm=isch&sa=X&ved=0ahUKEwiUhpG7hc7PAhVLrI8KHUtsDssQ_AUIBigB. Medicinal Pharmacology by K. D. Tripathi , 7 th edition, Jaypee Publication, Page no. 45-49. Principles of Pharmacology by HL Sharma and KK Sharma, 2 nd edition, Paras Publication, Pg. No. 252 REFERENCES: 10/22/2016 6

7. THANK YOU!!! 10/22/2016 7