Phytosomes

phytosomes

1. H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur Presented By: Pratik Anil Sonar M. Pharm 1 st year (Dept. of P’ceutics) Guided By : Dr. P. K. Deshmukh (Dept. of P’ceutics) “Phytosomes” 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 1

2. Content: Introduction Structure of phytosomes Strength of phytosomes Advantages Disadvantages Method of preparation Evaluation parameter Application Conclusion References 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 2

3. Introduction[1] The term “ phyto ” suggests that plant whereas “ somes ” suggests that cellular or cell like structure. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 3

4. Introduction cont … The Phytosome technology, developed by Indena S.P.A. of Italy . Phytosome is a patented technology (U.S. Patent). better absorbed and produces better results. Phytosomes are more bioavailable. Phytosome is novel drug delivery system is a that combines hydrophilic bioactive phytoconstituents of herbal extracts and bound by phospholipids . ( e.g. soybean phospholipids ,lecithin) 22/05/2020 Phytosomes prepared by pratik sonar MPH10 4

5. Structure of phytosomes PC ( Phosphatidylcholine ) is a bifunctional compound. Specifically the choline head (hydrophilic) binds to these compounds while the phosphatidyl portion (lipophilic) comprising the body and tail which then envelopes the choline bound material and forms phyto -phospholipid complex. Phosphatidyl -choline - phospholipid Phosphatidyl moiety - lipophilic Choline moiety - Hydrophilic 22/05/2020 Phytosomes prepared by pratik sonar MPH10 5

6. Strength of phytosomes [2] Strength of phytosomes Phytosomes show better stability as chemical bond is formed between phospholipid molecule and phytoconstituent(s). Dose of phytoconstituents is reduced due to more bioavailability of the phytoconstituents in the complex form. Duration of action is increased . Phytoconstituents complex with phospholipids are more stable in gastric sections and resist the action of gut bacteria. Enhanced permeability of phytoconstituents across the biological membranes . Absorption of lipid insoluble polar phytoconstituents through different routes shows better absorption, hence shows significantly higher therapeutic effects . Phoshatidylcholine used in the formation of phytosomes, besides acting as a carrier also possess several therapeutic properties, hence gives the synergistic effect when particular substance is given . Drug entrapment is not a problem with phytosome as the complex is biodegradable. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 6

7. Advantages of Phytosomes [2] Better bioavailability. Reduced dose. Enhanced absorption of herbal constituents. Better stability profile. Greater therapeutics benefit. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 7

8. Disadvantages of phytosomes [2] Phytoconstituent from phytosomes are rapidly eliminated. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 8

9. Method of preparation of phytosomes [1] Solvent evaporation methods Rotary evaporation technique Anti-solvent Precipitation Technique 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 9

10. 1)Solvent evaporation methods 22/05/2020 Phytosomes prepared by pratik sonar MPH10 10

11. 2)Rotary evaporation technique 22/05/2020 Phytosomes prepared by pratik sonar MPH10 11

12. 3)Anti-solvent Precipitation Technique 22/05/2020 Phytosomes prepared by pratik sonar MPH10 12

13. Evaluation of Phytosomes [3] Visualization: Visualization of phytosomes can be achieved using transmission electron microscopy (TEM) and by scanning electron microscopy (SEM). Vesicle size and zeta potential: The particle size and zeta potential can be determined by dynamic light scattering (DLS) using a computerized inspection system. Entrapment efficiency: The entrapment efficiency of a drug by phytosomes can be measured by the ultracentrifugation technique . 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 13

14. Transition temperature: The transition temperature of the vesicular lipid systems can be determined by differential scanning calorimeter (DSC). Surface tension activity measurement: The surface tension activity of the drug in aqueous solution can be measured by the ring method in a Du Nouy ring tensiometer . Drug content : The amount of drug can quantified by a suitable spectroscopic method . 22/05/2020 Phytosomes prepared by pratik sonar MPH10 14

15. FTIR: The spectroscopic evaluation of the formed complex can be confirmed by FTIR simply by comparing the spectrum of the complex and the individual components . In vitro and in vivo evaluations: Models of in‐vitro and in‐vivo evaluations are selected on the basis of the expected therapeutic activity of biologically active phytoconstituents present in the phytosomes . Spectroscopic evaluations: 1 HNMR 13 CNMR 22/05/2020 Phytosomes prepared by pratik sonar MPH10 15

16. Application[4] Enhancing Bioavalability: Evodiamine,a quinoline alkaloid, these pytosomes reduce the first-pass metabolism by passing liver and therefore avoiding the contact of the hepatic metabolism enzymes. Antioxidant properties: A phytosomal formulation of Quercetin was prepared with higher encapsulation efficiency and physical stability t o improve its efficacy in intestinal absorption and its preservation from oxidation in foodstuffs. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 16

17. Hepato -Protective: Andrographolide (AN) obtained from Andrographis paniculata linn include treatment of hepatic impairment. Cancer treatment: Silybin ( silibum marianum )is a natural polyphenol with antioxidant and anti-cancer properties which bloks estrogen receptor which are over expresses in breast cancer. 22/05/2020 Phytosomes prepared by pratik sonar MPH10 17

18. Wound healing: Ethanolic extract of wrightia arborea leaves and its phytosomes.the phytosome exhibited about 90.40%healing while the ethanolic extract alone could heal only 65.63%of the wound. Transdermal application: the phytosomal colpex of saponins and plant extracts ( panax ginseng M) more active in protection against UV radiation. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 18

19. Conclusion: Phytosomes forms a bridge between the conventional delivery system and novel delivery system. Phytosomes exhibit better pharmacokinetic and pharmacodynamic profile than conventional herbal extracts. Phytosomes is used to increase the solubility as well as permeability of the phytoconstituents present in part of the plant. 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 19

20. References: R . P. Singh, H. V. Gangadharappa , K. Mruthunjaya ; Phytosome Novel Herbal Drug Delivery System : A Review, International Research Journal Of Pharmacy; 2016, Vol 7, Issue 6. Amith Kumar B, Prasanna Habbu , Thimmasetty , Lakshman , Prabha Hullatti , Ravi Kumar S, Phytosomes as Novel Drug Delivery System for Herbal Medicine –A Review; Systematic Reviews in Pharmacy, Jan-Dec, 2017, Vol 8, Issue 1, 5-7. M. Sravanthi and J. Shiva Krishna, PHYTOSOMES: A Novel Drug Delivery For Herbal Extracts – A Review; International journal of Pharmaceutical science and research; 2013, Vol. 4, Issue 3, 949-959. Chivte Prajkta , Pardhi Vinal , Joshi Vineeta , Ajitha Rani R; A REVIEW ON THERAPEUTIC APPLICATIONS OF PHYTOSOMES; Journal of Drug Delivery and Therapeutics2017; 7(5):17-21 Josef kareparamban,a pravin novel revolution in herbal drugs ,IJRPC 2012,2(2) 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 20

21. Thank you 22/05/2020 Phytosomes prepared by Pratik sonar MPH10 21