PIT AND FISSURE SEALANTS 1.ppt

PIT AND FISSURE SEALANTS

1. Pit and fissure sealants
PIT AND FISSURE SEALANTS
DR.BASAVAN GOWDA
READER
DEPT.CONSERVATIVE
&ENDODONTICS
NAVODAYA DENTAL
COLLEGE,RAICHUR

2. Pit and fissure sealants
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3. Pit and fissure sealants
EVOLUTION OF PIT AND FISSURE SEALANTS
Wilsonused zinc phosphate
Kline and Knutson-use of ammoniacal silver nitrate
1922-“PROPHYLACTIC ODONTOTOMY” -HYATT
1939-Gore–use of polymers-sol. of cellulose nitrate

4. Pit and fissure sealants
1955-Buonocore–use of concentrated phosphoric acid
solution
Gwinnett and Buonocore-used 50% H3PO4 produced
porosity strong bond.
Cueto and Buonocore-used methyl cyanoacrylate monomer
filled with silicate filler-polymerization of cyanoacrylate and
releasing fluorides
1965-FIRST PAPERon pit and fissure published (Cueto
and Buonocore)
1968-Roydhouse –used BIS-GMA monomer using
methymethacrylate as diluent with peroxide amine
polymerization system

5. Pit and fissure sealants
1976-first colored sealant-CONCISE WHITE SEALANT
(3M dental products)
1984-Burt reported-“first and second molars should be
sealed as soon as possible after eruption because of their
susceptibility to occlusal caries”
1989-Eccles noted-“fissure sealant should be used
preventively for caries prone patient, and
therapeutically for suspect or early carious lesion

6. Pit and fissure sealants
DEFINITIONS:
Pit:isdefinedassmallpinpointdepressionlocatedatthe
junctionofdevelopmentalgroovesoratterminalsofthose
grooves.
Fissure:isdefinedasdeepcleftsbetweenadjoiningcusps.
Theyprovideareasforretentionofcariesproducingagents.
PitandFissureSealant:isusedtodescribea
materialthatisintroducedintotheocclusalpit
andfissureofcariessusceptibleteeth,thus
forming a micromechanically–bonded,
protectivelayercuttingaccessofcaries
producingbacteriafromtheirsourceof
nutrients

7. Pit and fissure sealants
HISTORIC DEVELOPMENT OF PREVENTIVE TECHNIQUES FOR
OCCLUSAL SURFACE:

8. Pit and fissure sealants
INEFFECTIVENESS OF FLUORIDE:
Systemic fluoride ingestion-selective benefit on smooth
surface caries
Thus, in fluoridated community-in smooth surface
caries relative in PF caries
Ingestion of fluorides in pre eruptive phase: enhances
coalescence of occlusal PF and reduces steepness of
cuspal inclines : in PF caries
SEALANTS + FLUORIDE = MAXIMUM PROTECTION
AGAINST CAVITIES

9. Pit and fissure sealants
EFFECTIVENESS OF SEALANTS:
Conservative preventive measure
When utilized in conjunction with water fluoridation, its
effectiveness increases by 20%
100% effective in protecting tooth surface
Retention varies for sealant coverage:
–96% after 1 yr.
–82% after 5 yrs
–57% after 10 yrs
–52% after 15 yrs
(JCPD Vol. 6: no.3: 2005)

10. Pit and fissure sealants
COST OF SEALANT PLACEMENT –
MINIMIZED BY –
Delegating treatment to auxilary personnel
Selecting commercial products with highest success
rates
Meticulous application procedure
Application of sealants in conjunction with fluorides
Proper patient selection

11. Pit and fissure sealants
HOW DO SEALANTS WORK ???
Keep substrates out of pits, fissures and grooves
Create an anaerobic environment -eliminate the
aerobic bacteria and other decaying matter
residing in this area of the tooth.

12. Pit and fissure sealants
MICROFLORA OF PIT AND FISSURES —
Cocciconstitute –75% to 95%of microorganisms
S.Sanguis–Predominant viable microorganisms
S.Mutans and Lactobacilli –
•low in newly formed plaque in fissures
•over time
Fusiforms, Spirillae and Spirochetesare
absent

13. Pit and fissure sealants
PLAQUE COMPOSITION IN FISSURES :
Fissures contain microorganisms
and food particles
Limited morphological types
occur in fissure
Palisading and branching
filaments are absent within
fissures but may colonize at
orifice
Empty ghost-like cell wall
structures intermingle with viable
cells in some areas

14. Pit and fissure sealants
CLASSIFICATION OF SEALANTS –
BASED ON GENERATION –
A.First generation sealants:
-polymerized by UV-light at a wavelength 356 μm.
-disad.: -Excessive absorption and incomplete
polymerization of sealant at its depth.
-variable output intensity
-output not uniform
B. Second generation Sealants/Self curing resins
-Based on catalyst –accelerator system
-Most are unfilled.
-May be transparent, tinted or opaque by inclusion of
white pigment or a tint for better visualization.
eg . Concise [3M] white sealant system, Delton.

15. Pit and fissure sealants
C.Third generation sealants:
-Lightcuredbyvisiblelightatwavelength430nm-490nm.
-Maybeclassifiedasfilledorunfilled,andwithorwithout
tintoropaquer.
-Mostoftheunfilledresinsarecoloredwhite.
-Filledresinsareeitherclear,yellowishwhiteortan
PERFORMBETTERTHANSELFCURERESINS
D.Fourth generation sealants:
-Are those containing fluorides.

16. Pit and fissure sealants
FLUORIDES INCORPORATION IN FISSURE SEALANTS
Fluoride is added to unpolymerized resin in form of
soluble salt
-Releases fluoride for extended period: 24 hrs. to 30
days
An organic fluoride compoundis chemically bound to
resin
-An ion from saliva diffused into resin , exchanged
with fluoride ion –which then is diffused out and is
released
FLUORIDE REPLACED RATHER THAN LOST

17. Pit and fissure sealants
BASED ON FILLER CONTENT:
1.Unfilled [ free of fillers ]
-flow is better
-retention is more
-abrade rapidly
eg . Concise White
2. Filled
-need for occlusal adjustments
-more resistant to wear
eg. Prisma shield

18. Pit and fissure sealants
BASED ON TRANSLUCENCY :
1.CLEAR
-Esthetic, but difficult to detect at recall examination.
-Better flow than tinted or opaque
-More easily appreciated by the patient.
2.TINTED / OPAQUE
-can be easily identified
–COLOURED
-easy to see during placement
-easy to see during recall check up

19. Pit and fissure sealants
BASED ON CURING:
1. AUTOPOLYMERIZING.
-Better retention 88%
-Sets by exothermic reaction
2.LIGHT CURE
-75% retentive

20. Pit and fissure sealants
MATERIALS USED AS PIT AND FISSURE SEALANTS
•DEVELOPMENT OF CYANOACRYLATES:polymerized to hard but
brittle polymers
–Disad: bond to unetched enamel is poor
material sticks to skin
mechanical durability poor
biodegradable
hydrolysis of cyanoacrylates to toxic materials
•Recent cyanoacrylates –butyl and isobutyl esters
•Cyanoacrylates mixed with fluoride containing fillers are
also available

21. Pit and fissure sealants
•POLYURETHANES :
•Di-isocyante + high mol. wt glycol
•Urethane prepolymer
•High mol. wt elastomer
•Adhesion of these polyurethanes to enamel is not
satisfactory
•Eg. EPOXYLITE 9070, ELMEX PROTECTOR
•Disad : poor mechanical properties
low oral durability

22. Pit and fissure sealants
•Enamite –utilizes methyl methacrylate -poly methyl methacrylate
initiated by butyl boron
•BOWEN: INTRODUCED BIS-GMA-SEALANT OF CHOICE
•Addition of BIS-PHENOL A and GLYCIDYL METHACRYLATE
BIS-GMA
•In 1972, Nuva-Seal was the first successful commercial sealant to be
used.
•Hydroxyl group in BIS-GMA is responsible for viscosity
•Some of these contain fillers, which makes it desirable to classify the
commercial products into filled and unfilled sealants.
•The fillers make the sealant more resistant to abrasion

23. Pit and fissure sealants
RATIONALE FOR USE OF SEALANTS-
Safe, effective and underused in preventing PF caries on
at-risk surfaces. Effectiveness-increased by follow up
Benefit increased by placement on high risk surfacesor
surfaces that already exhibit incipient carious lesions
Best evaluation of risk-made by experienced clinician
Sealant benefit-exist in any tooth with pit and fissure

24. Pit and fissure sealants
Sealant placement-careful cleaning without
removing any enamel
Placement of low viscosity, hydrophilic
material under sealant-enhances retention
and effectiveness
GIC is shown to be ineffective as PFS
Awareness-new preventive methods effective
against PF caries.

25. Pit and fissure sealants
CARIES RISK ASSESSMENT:
WIDER EXAMINATION TO MAKE AN ASSESSMENT OF OVERALL CARIES
RISK
Time Since Tooth Eruption:
General condition of the mouth:
Age of the patient:
Attendance record of the patient:
Medical conditions:

26. Pit and fissure sealants
DIAGNOSIS OF PITS AND FISSURES
•Diagnosis-based on tactile evaluation with an
explorer and visual assessment of the enamel
appearance
•Clinical examination varies highly from one
practitioner to another -owing to the size and
shape of the explorer tip, the force applied, and
the judgment of the examiner.
•Radiographic evaluation of occlusal surfaces-
minimal diagnostic value

27. Pit and fissure sealants
INDICATIONS :
–RISK-BASED SEALANT TREATMENT
Possibility of adequate isolation
Questionable enamel caries in PF
Xerostomia
Patients undergoing orthodontic treatment
Deep pits and fissures

28. Pit and fissure sealants
CONTRAINDICATIONS ;
Posterior teeth that have shallow or well coalesced
fissures
Low caries risk (PF that remained caries free > 4 yrs)
Rampant caries
Teeth with proximal decay or occlusal caries involving
dentine
Allergy to methacrylate
Semi-erupted teeth

29. Pit and fissure sealants
REQUIREMENTS FOR OCCLUSAL SEALANTS :
•Non-toxicandnonirritatingtotissues.
•Adheretothetoothasathinlayerandforan
extendedperiodoftime.
•Theconsistencyandviscosity-permitflowand
penetrationeasilyintosmallareas
•Mechanical,compressiveandtensileproperties
ofthematerial-sufficienttowithstand
masticationandresistwear.

30. Pit and fissure sealants
•Water absorption -low, thus-resistance to
displacement and discoloration.
•Should be able to be seen during application and
at recall appointments
•Low solubility in oral fluids.
•Cariostatic action

31. Pit and fissure sealants
TYPES OF OCCLUSAL FISSURES:
V–Wide at top and gradually narrowing towards the
bottom (34%)
U–Almost same width from top to bottom (14%)
IK–Hourglass, extremely narrow slit associated with a
large space at the bottom (26%).
λ–Inverted Y, bifurcating at the bottom (7%).
I–Extremely narrow slit (19%).

32. Pit and fissure sealants

33. Pit and fissure sealants
MORPHOLOGY OF SURFACES WITH PITS
AND FISSURES:
Caries in PF-related to form and depth of these P and F
Two main type of pits and fissures are usually described
1)Shallow, wide v-shaped fissures-self cleansing and CARIES
RESISTANT
2)Deep, narrow I-shaped fissures-constricted and resemble bottle
neck. May have different branches-CARIES SUSCEPTIBLE
PF vary in shape-0.1 mm wide and tortuous

34. Pit and fissure sealants
Fissure contains an organic plug composed of reduced
enamel epithelium, microorganisms forming dental
plaque and oral debris
Fissure provides a niche for plaque accumulation
Morphology of occlusal surfaces varies from one tooth
to another-
1)Premolar-prominent primary fissure with 3 or 4 pits
2) Molar-as many as 10 separate pits may be present in primary,
secondary and supplemental fissures
Dental caries in fissures:

35. Pit and fissure sealants
Enamel rods flare laterally in bottom of PF
Caries occur
Follow direction of enamel rods
Triangular lesion with base toward DEJ
Greater no. of dentinal tubules involved
Pit and fissure caries (occlusal surfaces) involve greater cavitation than
proximal lesions

36. Pit and fissure sealants
HISTOPATHOLOGY OF CARIES IN PITS AND FISSURES:
Initially, caries progress in fissures-thought to begin at
base of fissure
Inclines forming walls of fissure are affected first by
caries process
•CARIES PROGRESS IN PF HISTOLOGICALLY CAN BE
EXPLAINED AS FOLLOWS:

37. Pit and fissure sealants
First evidence of lesion formation occurs at orifice of
fissure-two independent bilateral lesions
Depth of fissure walls become involved
Coalescence of two independent lesions into single,
contiguous lesion
Once caries involves dentin-progress is enhanced
Cavitation of fissure-loss of mineral and structural support
Clinically detectable lesion

38. Pit and fissure sealants
Unique process of caries formationin fissure-
–Presence of organic plug in fissure acts as a buffer
against acid byproduct of plaque provides a
diffusion barrier lessened acid attack at fissural
base during initial phase of caries formation
Base of fissure may be close to or lie within dentin
dentin involvement is rapid frank cavitated lesion
•BUT ON SMOOTH SURFACES -

39. Pit and fissure sealants
Atleast 1mm of enamel present superficial to DEJ
in smooth surfaces
Sufficient enamel has to become involved to reach
dentine
3-4 yrs reqd -dentinal involvement
Remineralization of caries may occur on exposure
to fluorides
Reversal of lesion may occur

40. Pit and fissure sealants
TECHNIQUE FOR SEALANT APPLICATION –
PREPARATION OF TOOTH
ISOLATION
DRYING THE TOOTH
ETCHING OF TOOTH SURFACE
RINSING AND DRYING OF TOOTH
PLACEMENT AND POLYMERIZATION OF SEALANT
OCCLUSAL EVALUATION

41. Pit and fissure sealants
Tooth preparation :
Earlier -cleaning enamel surface with pumice and water
mixture using rotary brush
By patient-Direct bristles of dry brush in PF
Use of explorer
Use of Prophy-Jet: air polishing system
Air abrasionsystem with 50 um alumina
Mechanical preparationof fissure with tapered fissure
diamond bur -retention
TECH. FOR SEALANT APPLICATION

42. Pit and fissure sealants
•Isolation of teeth :
SALIVA CONTAMINATION AVOIDED TO PREVENT REMINERALIZATION OF
ETCHED SURFACES
Acc to Ferguson and Ripa–rubber dam
isolation provides better retention rates for
UV-light activated sealants
Use of cotton rolls–when using
autopolymerized resins
Rubber dam isolation-used when a
quadrant is to be isolated
TECH. FOR SEALANT APPLICATION

43. Pit and fissure sealants
•Etching :
Applied using–small sponge, cotton pellet or brush may
be used
Etchant available as –liquid, gel or semi-gel form
“SKIPPING EFFECT”-USE OF GEL ETCHANT
Concentration used–30 –40%
TECH. FOR SEALANT APPLICATION
Technique of application: continuous
but gentle dabbing or agitation of sol on
enamel surface
Rubbing

44. Pit and fissure sealants
Site of application–2/3 rd way up cuspal slopes
Etch approx. 2 mm on either side of an exposed groove
Primary enamel has prismless structure –but it is not
found on occlusal surface
Shorter etching time for primary molars -chances of
contamination, during etching (acceptable for 3-4 yr old
children)
Etching timehas no effect on sealant retention
TECH. FOR SEALANT APPLICATION

45. Pit and fissure sealants
•WHYISPROLONGEDETCHINGTIMENECESSARYFORPRIMARY
TEETH???
PrimaryenamelhasLOWMINERALCONTENTand
HIGHINTERNALPOREVOLUME–
moreexogenousorganicmaterialthandoespermanenttoothsurface–
hencedecidingetchingcharacteristicsofdeciduousenamel

46. Pit and fissure sealants
PATTERNS OF ETCHING –
•TYPE 1: generalized roughening of enamel
surface but with distinct hollowing of prism
centers and intact peripheral margins
•TYPE 2: peripheries are heavily damaged and
cores are left
damage of periphery extends to whole length-
delineates prism
•TYPE 3: generalized
roughening

47. Pit and fissure sealants
ZONES OF ETCHING
•NARROW ZONE OF ENAMEL/ETCHED ZONE –10u
deep that is lost by etching
•QUALITATIVE POROUS ZONE –20u deep, rendered
porous-as seen in polarizing microscope
•QUANTITATIVE POROUS ZONE/DEEPER ZONE –
20u thick –indistinguishable from sound enamel, but is
slightly porous due to acid

48. Pit and fissure sealants
Washing and drying :
REMOVE ALL ACID AND REACTIONARY PRECIPITATES
Rinse with water for 10-20 seconds and dried for additional
10 sec
Water under pressure in air-water spray + high power
evacuation
Evacuator tip placed above/adjacent the tooth and water
directed to the tip
20 sec/tooth OR 30 sec/quadrant
TECH. FOR SEALANT APPLICATION

49. Pit and fissure sealants
•If contamination occurs . . . .
If cotton rolls are being used-replaced after becoming
saturated during etching and washing
If sal cont. does occur –re-etching for 10 sec before
washing once again
If etched enamel is exposed to sal for 1 to 60 secre-
etching
Minimal sal exposure for less than 10 secimmediate
washing performed
TECH. FOR SEALANT APPLICATION

50. Pit and fissure sealants
Use of dentin bonding agents . . .
Feigal et al---hydrophilic bonding materials..
may, when applied under sealant, minimize
the bond strength normally lost --when a
sealant is applied in a moist environment
TECH. FOR SEALANT APPLICATION

51. Pit and fissure sealants
Bonding agentunder sealant on wet contamination–
Bonding agentused without contamination–
It also reduces microleakage
In primary teeth-use of sealants in moisture
contaminated areas gave better results than sealant
alone on non-contaminated areas
SYMON PROPOSED :
Single bottle agents-protect sealant survival :
•Yeild half the usual risk of failure for occ sealants
•Yeild 1/3 the risk of failure for buccal/lingual sealants
TECH. FOR SEALANT APPLICATION

52. Pit and fissure sealants
Sealant application --
Sealant applied with disposable bristle brush
For autopolymerizing resins–cover etched areas on each tooth as
quickly as possible with sealant and then bulk can be added
For light curing resins–no mixing necessary and hence reduced
bubbles
Sealant applied should be –
–too much
–thick
THUS TOTAL TIME FOR SEALANT APPLICATION SHOULD TAKE
3 ½ MIN
TECH. FOR SEALANT APPLICATION

53. Pit and fissure sealants
Adjustment and recall –
Surface wiped off to remove surface film –
inspection of surface
Occlusion checked with articulating papers
Occlusal interferences removed
•Filled sealants adjusted with green stone
Sealants should be evaluated every 6 mnths
•Bite-wing RG: detect caries progression under
sealants
TECH. FOR SEALANT APPLICATION

54. Pit and fissure sealants
LAST STEP OF SEALANT APPLICATION INVOLVES EDUCATION
OF PATIENT AND THE PARENTS ABOUT THE IMPORTANCE
OF PERIODIC RE--EVALUATION OF THE SEALANTS
TECH. FOR SEALANT APPLICATION

55. Pit and fissure sealants
CLINICAL PROBLEMS WITH SEALANT USE:
Lack of universal usage:
•Not every patient should receive sealant therapy
•To achieve greatest possible caries reduction -use sealants
in conjunction with syst./topical fluorides, sound dietary
habits and good oral hygiene
•Patients selected for PFS placement must-
–Dependable on recall appts.
–Motivated and proficient in caries control
–Low caries activity
–Receive syst/topical fluorides

56. Pit and fissure sealants
•Acc.to Simonsen-both caries free and caries
rampant pt-left unsealed
•Highly cost effective method-delay sealant
placement until first evidence of caries
•Acc. toRipa: All children –potential candidates
for sealant placement
•Effectiveness affected by tooth type selected
CLINICAL PROBLEMS

57. Pit and fissure sealants
TOOTH ORIENTED INDICATIONS AND CONTRAINDICATIONS FOR USE
OF PFS
SURFACE DIAGNOSISCLINICAL
CONSIDERATIONS
DO SEAL DO NOT SEAL
CARIOUS 1.Occlusal anat1.If PF separated
by transverse
ridge-sound PF
1.Carious PF
QUESTIONABL
E
1.Status of prox
surfaces
2.General caries
activity
(1.Sound
2.Any occ lesions,
few prox lesions)
1.Carious
2.Many prox
lesions
SOUND 1.Occ morph
2.Tooth age
3.Status of prox
surf
4.General caries
activity
1.Deep, narrowPF
2.Recently
erupted
( )
1.Broad well
coalesced fiss
2.Teeth caries
free for >4 yrs
3.Many prox
lesions

58. Pit and fissure sealants
Technique sensitivity:
•Strict adherence to manufacturer’s recommendation for
placement of sealant.
•Quality of isolation
•Difficulty in partially erupted teeth: twice sealant loss
than in completely eruptedmolars
CLINICAL PROBLEMS

59. Pit and fissure sealants
Caries susceptibility of etched enamel:
•Nonsealed, etched surfaces-not more susceptible to caries
•Etching on cuspal inclines
•Etched enamel remineralizes completely within 48 hrs
•Occlusal sites that have lost sealant coverage-not
susceptible to future caries devlpt because of etching
CLINICAL PROBLEMS

60. Pit and fissure sealants
Detection of lost sealant:
•Opaque colored sealants are more visually detectable
•1976-first commercial colored sealant was launched
•Chief criticism –
•26% of sealants placed after 4 yrs illustrated marginal
discoloration and stain penetration
CLINICAL PROBLEMS

61. Pit and fissure sealants
LOSS OF SEALANT

62. Pit and fissure sealants
Inadvertent placement over carious sites:
•Not recommended over detectable carious lesion-unless
marginal integrity can be maintained
•Acc to some studies-reduction in viable organisms
cultured from sealed fissures
•Inactivity was also seen by-lack of RG progression
observed over time
•SOME BACTERIA REMAIN VIABLE AND PRESUMABLY RETAIN
THEIR POTENTIAL FOR PATHOGENICITY
CLINICAL PROBLEMS

63. Pit and fissure sealants
POTENTIAL PROBLEMS AND ITS SOLUTIONS IN SEALANT PLACEMENT --
PROBLEM ITS SOLUTION
1. Air bubbles-present
under/bet sealants
Sealant should be ground to expose
bubble-refill with same sealant material
2. Porosities in sealantReapply sealant
3. Loss of sealant Reapply sealant-they are not permanent
Some protection provided even when
they are lost-due to sealant present in
microporosities created by etching
4. Decay present under
sealant
If excessive-remove sealant and place
restoration or PRR
If minute decay left-not a problem, as it
creates an environment in which bact
wont thrive

64. Pit and fissure sealants
RISK FACTORS FOR SEALANTS --

65. Pit and fissure sealants
CHILDREN RISK FACTORS
1.LOW RISK •No new/incipient caries in past year
•Good oral hygiene
•Regular dental visits
2.MODERATE
RISK
One new, incipient/recurrent caries in past year
Deep PF
High familial caries experience
Early childhood caries
Frequent sugar exposure
Decreased salivary flow
Compromised oral hygiene
Irregular dental visits
Inadequate fluoride exposure
3.HIGH RISKTwo or more new or recurrent carious lesionsin past year
Deep PF
Sibling or parents with high caries rate
History of pit and fissure caries
Early childhood caries
Frequent sugar exposures
Decreased salivary flow

66. Pit and fissure sealants
PREVENTIVE RESIN RESTORATIONS (PRR)–
FISSURE SEALANT OR SEALANT RESTORATION
•It is a natural extension of Pit and Fissure
sealants.
•INDICATIONS
–Tooth can be isolated.
–No, or only minimal pit and fissure staining
–Minimal “catches” in the grooves, or areas with
distinct incipient enamel caries.
–No evidence of radiographic caries.

67. Pit and fissure sealants
•Three types of PRR–based on extent and
depth of carious lesion as determined by
exploratory preparation.
TYPE A: suspicious PF where caries
removal limited to enamel
TYPE B: incipient lesion in dentin that is
small and confined
TYPE C: is characterized for greater
exploratory preparation in dentin

68. Pit and fissure sealants
TYPE OF
SEALANT
RESTORATION
INDICATIONS
1. Sealant aloneDecalcified fissure
No RG involvement of dentin
Less than 2 other carious lesions in mouth
2. Composite +
Sealant
More than 2 other carious lesions in mouth
Lesion confined to dentin
3. GIC + SealantCavity in dentin but confined
Margins not in occlusal contact
4. Laminate
restoration
Lesion in dentin and lateral spread along DEJ
Cavity margins in occlusal contact
5. Amalgam
restoration
Large radiolucency in dentin
Significant lateral spread of caries

69. Pit and fissure sealants
•RECENT ADVANCES :
•Use of surfactant containing etchant–lower surface
tension and contact angle
•Use of argon laser for polymerization
•Use of Er: YAG laser
•Carbon dioxide conditioning
•Use of DIAGNOdent –detection of caries
under pit and fissures