Pract no-1 MIcroscopy didcripation practical

Microscopy practical

1. Clinical PathologyClinical Pathology
Practical No. IPractical No. I

2. Components of compound microscopeComponents of compound microscope

6. Light pathLight path

7. 
Image Path- Object to eye pieceImage Path- Object to eye piece

10. Dark ground illuminationDark ground illumination..

In DGI oblique rays of light illuminate In DGI oblique rays of light illuminate
the edges of object by using a special the edges of object by using a special
type of condenser and only type of condenser and only light light
gatheredgathered is is reflected reflected or or diffracteddiffracted by by
structures within the specimen.structures within the specimen.

Example – micro-organisms Example – micro-organisms
(spirochetes) (spirochetes)

15. LeptospiraLeptospira

16. Crystals- Uric acidCrystals- Uric acid

17. SpirochetesSpirochetes

18. Phase contrast microscopyPhase contrast microscopy

Specimen retards some light rays with Specimen retards some light rays with
respect to those which pass through respect to those which pass through
surrounding mediumsurrounding medium

A special type of condenser , objective A special type of condenser , objective
and and phase- discphase- disc

Examples –living cells , crystals and Examples –living cells , crystals and
fibres.fibres.

22. Epithelial CellEpithelial Cell

23. Trichuris trichuraTrichuris trichura

24. Epithelial cellsEpithelial cells

25. CiliaCilia

26. Fluorescence microscopyFluorescence microscopy
Fluorescence –is the property of some Fluorescence –is the property of some
substances which when illuminated by light of substances which when illuminated by light of
certain wave length will re-emitcertain wave length will re-emit
the light at a longer wave length.the light at a longer wave length.
Auto fluorescenceAuto fluorescence
Secondary fluorescence- dyes like auramine , Secondary fluorescence- dyes like auramine ,
rhodamine and thioflavinerhodamine and thioflavine
Immunofluorescence –to demonstrate the AG.Immunofluorescence –to demonstrate the AG.
Eg: amyloid.Eg: amyloid.

29. GlomerulusGlomerulus

30. Cultured cellsCultured cells

31. Polarizing microscope.Polarizing microscope.

Polarization- is a method of converting normal Polarization- is a method of converting normal
light into a light having waves with light into a light having waves with
electromagnetic vibrations in only one of the electromagnetic vibrations in only one of the
direction perpendicular to the wave motion.direction perpendicular to the wave motion.

PolarizerPolarizer

Birefringence - is a property of object to Birefringence - is a property of object to
convert a polarize light into two different light convert a polarize light into two different light
(color).(color).

Example-amyloid, sphingomyelin. Example-amyloid, sphingomyelin.

35. Kidney AmyloidKidney Amyloid
BirefringenceBirefringence

36. Amyloid Amyloid
Apple green birefringenceApple green birefringence

37. Compact boneCompact bone

38. Electron microscopyElectron microscopy

Great Great resolution resolution and and magnificationmagnification

Electron gun acts as a source of lightElectron gun acts as a source of light

Electromagnetic field acts as lenses.Electromagnetic field acts as lenses.
1.Transmission E.M.1.Transmission E.M.
2.Scanning E.M.2.Scanning E.M.
3. Analytical E.M.3. Analytical E.M.

Fixation- osmium tetraoxide, uraniumFixation- osmium tetraoxide, uranium..

41. SEM- RBC & WBCSEM- RBC & WBC

42. SEM -ChromosomeSEM -Chromosome

43. SEM- RetinaSEM- Retina

44. SEM- TongueSEM- Tongue