Presentation senna

Presentation on senna plant and its characetristics, chemical constituencies and more

1. SENOSIDES Presented by Tulsi ram jabegu M.Pham 1 st (sem.Pharmacognosy and phytochemistry ) Submitted To .Dr M.Mujeeb M. Pham. PhD Department of Pharmacy JAMIA HAMDARD New Deilhi

2. Contents Introduction Characteristics of sennosides Chemical structure of sennosides Sennosides contains various parts of senna plant Biosynthesis of sennosides Extraction and isolation method of sennosides Identification of sennosides Purification methods References

3. Sennoside   S ennoside is known as senna glycoside  or  senna , is a medication used to treat  constipation  and empty the  large intestine  before surgery .  The medication is taken by mouth or via the  rectum .  It typically begins working in minutes when given by rectum and within twelve hours when given by mouth .  It is a weaker laxative than  bisacodyl  or  castor oil .

4. Characteristic of sennosides Mol .formula C42H38O20 mol. Wt 862.746g/ mol M.p . 329f it is brown powder slight odour and taste solid (9R)-9-[(9R)-2-carboxy-4-hydroxy-10-oxo-5-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-9H-anthracen-9-yl]-4-hydroxy-10-oxo-5-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-9H-anthracene-2-carboxylic acid 2D Structure of sennosides

5. Chemical structure of sennosides Sennosides are obtained from the dried leaflets of Cassia senna (Cassia acutifoliaj Delile and Cassia angustifolia Vahl (Fam: Leguminosae).

6. Chemical structures cont …

7. Sennoside B content in various parts of C. angustifolia. Part Sennoside B content % Filament and Anthers 7.2 Carpels and ovaries 5.8 Petals 5.2 Sepals 4.7 Flowers 4.3 Flower stalk 3.2 Leaflets 2.8 Pericarp 2.4 Stems 0.2 Roots 0.05

8. Cont…

9. Purification of Sennosides

10. Purification cont.… HPLC : a. Meta . hod : isocratic method b. Mobile phase :ethanol:water:acetic acid:tetrahydrofuron . (60:38:2:2). c. Detection:25 μ nm. Further purification can be done by: Column chromatography GC-MS

11. References www.science direct .com. www.slide share.com www.pubmed.com Google image