Preservation and storage

students will be benefited

1. BLOOD PRESERVATION AND STORAGE

2. BLOOD PRESERVATION AND STORAGE BLOOD FOR TRANSFUSION MUST BE COLLECTED INTO AN ANTOCOAGULANT COMMONLY USED ANTOCOAGULANT IS SODIUM CITRATE CERTAIN SITUATIONS IN PREFERENCE TO CITRATE HEPARIN IS MORE EXPENSIVE (ONLY 24-48 HOURS) 15-30mg OF HEPARIN MIXED WITH 25ml OF NORMAL SALINE,SHOULD BE USED FOR A 500ml ‘UNIT’

3. BLOOD PRESERVATION AND STORAGE SOLUTION,WHICH GIVES BOTH ANTICOAGULATION AND PRESERVATION IS A MIXTURE OF CITRIC ACID,SODIUM CITRATE AND DEXTROSE(GLUCOSE) - ACD(ACID CITRATE DEXTOSE) SOLUTION

4. BLOOD PRESERVATION AND STORAGE DEXTROSE - NOURISHMENT FOR THE CELLS METABOLISM IS SLOWED DOWN AT LOW TEMPERATURE SURVIVAL OF THE CELLS,NOT ONLY IN THE BOTTLE,BUT ALSO IN THE BLOOD OF THE RECIPIENT AFTER TRANSFUSION,IS PROLONGED. ACD SOLUTION STORED FOR 21 DAYS ACD SOLUTION IS PREPARD AS FOLLOWS:

5. NIH FORMULA A NIH FORMULA B TRISODIUM CITRATE 22.0g 13.2g CITRIC ACID 8.0g 4.8 g DEXTROSE 24.5g 14.7g DISTILLED WATER 1000ml 1000ml BRITISH FORMULA DISODIUM CITRATE (monohydrate) 2g DEXTROSE 3g DISTILLED WATER 120ml

6. BLOOD PRESERVATION AND STORAGE USUALLY RECOMMENDED PROPORTION OF THESE FORMULAE USED PER 100ml OF BLOOD ARE: NIH FORMULA A 15ml NIH FORMULA B 25 ml BRITISH FORMULA 28.5ml

7. BLOOD PRESERVATION AND STORAGE BLOOD BANK WHICH PREPARE THEIR OWN BOTTLES USE NIH FORMULA A 80 ml OF ACD SOLTION FOR A 500ml ‘UNIT’ PROPER AMOUNT OF THE SOLUTION IS PLACED IN A CLEAN BOTTLE SEALED AND AUTOCLAVED IMMEDIATELY BLOOD BANK PURCHASES ALREADY PREPARED BOTTLES OR PLASTIC BAGS SHOULD CHECK THE LABELS TO DETERMINE THE TYPE AND THE VOLUME OF THE SOLUTION USED

8. BLOOD PRESERVATION AND STORAGE CPD(CITRATE PHOSPHATE DEXTOSE) LESS ACID SLIGHTLY LONGER PRESERVATION OF BLOOD COMPOSITION OF CPD: SODIUM CITRATE 2.63 g CITRIC ACID MONOHYDRATE 0.327g DEXTROSE MONOHYDRATE 2.55 g DISTILLED WATER 100ml MINIMUM 14ml OF CPD SOLUTION IS USED FOR 100ml OF BLOOD CPD SOLUTION CAN BE USED FOR LONGER DAYS CPD – A SOLUTION CAN BE UP TO 28 DAYS

9. BLOOD PRESERVATION AND STORAGE SOLUTION IN SOME OF THE PLASTIC BAGS AVAILABLE COMMERCIALLY FOR COLLECTION OF BLOOD HAS AN EVEN NEWER ANTICOAGULANT – PRESERVATIVE SOLUTION WHICH IS CALLED CPD –A SOLUTION CPD SOLUTION TO WHICH ADENINE HAS BEEN ADDED,TO GIVE BETTER PRESERVATION OF THE BLOOD CELLS CPD-A SOLUTION SODIUM CITRATE (DIHYDRATE) 2.63g CITRIC ACID (ANHYDROUS) 0.3g MONOBASIC SODIUM PHOSPHATE 0.22g DEXTROSE (MONOHYDRATE) 3.19g ADENINE(ANHYDROUS) 0.0275g DISTILLED WATER 100ml 14ml/100ml of blood Bags -49ml CPD –A SOLUTION FOR COLLECTING 350ml OF BLOOD

10. BLOOD PRESERVATION AND STORAGE BLOOD FOR TRANSFUSION SHOULD BE STORED AS 4-6°C IN A SEPARATE ‘BLOOD BANK’REFRIGERATOR SHOULD BE STORED IN A REFRIGERATOR IN WHICH THE DOOR IS NOT BEING COMPLETELY OPENED AND CLOSED FREQUENT OPENING AND CLOSING OF THE DOOR LEADS TO TEMPERATURE FLUCTUATIONS WHICH WILL DECREASE THE SAFE DURATION OF STORAGE AUTOMATIC ALARMS SET TO RING IF THE TEMPERATURE RISES ABOVE 8-10 °C

11. PREVOIUS YEAR QUESTIONS NAME 2 ANTICOAGULANT USED IN BLOOD BANK? AUGUST 2013