Proteomics.pptx

protein

1. PROTEOMICS PRESENTED BY HINA YASMEEN ROLL#19011506-060 PRESENTED TO DR.KHIZAR HAYAT BHATTI

2. Proteome defines the complete set of proteins expressed during a cell’s entire lifetime. Proteomics is the study of the proteome; it uses technologies ranging from genetic analysis to mass spectrometry. Proteomics assesses activities, modifications, localization, and interactions of proteins in complexes.

3. GENOMICS TRANSCRIPTOMICS PROTEOMICS

4. Proteome indicates the total proteins expressed by a genome in a cell or tissue. Biomarkers detection might allow identification of patients who would benefit from further evaluation. With the development of proteomic techniques, proteome analysis provides a fast, non-invasive d iag no s t ic to o l for patients wi t h v ar i ou s disea s es. The advent of highly sensitive proteomic technologies can identify proteins associated with development of diseases well before any clinically identifiable alteration. MS has a high resolving power and identifies proteins with more accuracy.

5. 1. Structural Proteomics :- The ultimate aim of this proteomics is to build a body of structural information that will help predict the probable structure and potential function for almost any protein from knowledge of its coding sequence. Functional proteomics :- It refers to the use of proteomics techniques to analyze the characteristics of molecular protein-networks involved in a living cell. Expression proteomics :- It refers to the quantitative study of protein expression between sample differing by some variable.

6. Separation of proteins One dimentional electrophoressis 2 D electrophossis(modern) Multi-dimensional HPLC (modern) Analysis of proteins Mass Spectrometry (modern) Database utilization

7. Sample collection, handling and storage. Separat i on o f i ndiv i dual pro t eins b y 2 - D electrophoresis. Protein characterization. Ident i fication b y ma s s spectrom e t ry o r ot h er methods. Storage, manipulation, and comparison of the data using bioinformatics .

8. The large-scale analysis of protein Protein structural comparisons can help to identify the function of newly discovered genes X-ray crystallography, NMR spectroscopy.

9. 1 one – Dimensional SDS-PAGE 2 Two- Dimensional SDS-PAGE What is SDS-PAGE? – SDS-PAGE a type of gel electrophoresis. What is the purpose of doing gel electrophoresis? – It has been seen that by running a gel we are able to identify more proteins from the sample. An electric current is applied across the gel, causing proteins will differentially migrate based on their molecular mass .

10. 2-D gel electrophoresis a method for the separation and identification of proteins in a sample by displacement in 2 dimensions. First step is to separate based on charge or isoelectric point, called isoelectric focusing. Then separate based on size (SDS-PAGE).

11. Application of Proteomics Protein sample identification/ confirmation. Protein sample purity determination. Detection of post-translational modifications Detection of amino acids substitution. Mass fingerprint identification of proteins. Nutrition Research To identify unknown protein of intrest. Quantify protein and peptide. Protein Biomarker.