Ps

Peripheral Smear (PS)
Smear: Layer of blood.

1. PS- Peripheral Smear Ms Ankita R Bhatiya Assistant Professor Shree P.M.Patel COLLEGE OF PARAMEDICAL SCIENCE N TECHNOLOGY

2. It include: 1.What is smear? 2.Purpose of making smear. 3.Types of smear

3. PS- Peripheral Smear Peripheral Smear (PS) Smear: Layer of blood. PS is performed for following purpose : 1.For observe morphology of RBC & WBC. 2. To check any abnormality in RBC & WBC. 3. For the detection or various types or anaemia & Leukaemia. 4. To Check Parasitic infection.

4. PS- Peripheral Smear There are two types of smear. 1.Thin 2. Thick. 1.Thin Smear: Four major steps involve in Thin Smear. 1. Preparation of Smear 2. Fixation of Smear 3. Staining of Smear 4. Microscopic Examination

5. PS- Peripheral Smear 1.Preparation of Smear Requirement: Slide, Spreader slide, dropper. Sample: EDTA blood sample or capillary blood. Procedure: 1. Take a clean and dry slide. 2.Transfer a drop of blood near the edge of the slide. 3. Put a spreader-slide at 30 to 35 angle. 4. Pull back the spreader slide until it touches the blood drop. 5.Push the spreader in forward direction with smooth movement. 6. A thin layer is formed which appear in Three zone. 7. Dry the blood smear at room temp. Adequate drying is essential to preserve the quality of the film.

6. PS- Peripheral Smear

7. PS- Peripheral Smear 2.Fixation of Smear: Requirement: Methanol Procedure: 1. Fix the blood smear with methanol for 2-3 min to prevent distortion of cells. 2. Keep it for air dry.

8. PS- Peripheral Smear 3.Staining of smear: Stain: It is one type of chemical which give colour to blood cell and organism. Following stain which are used for staining of smear. 1. Polychromatic stain 2. Romanowasky stain 3. Leishman stain 4. Field stain (field A, field B) Polychromatic stain: - Stain contains more than one dye. Ex: Giemsa Stain it's contain Methylene blue basic dye and Eosin acidic dye Eosin stain basic component of cell like cytoplasm. Methylene blue stain acidic component of cell like nucleus. Neutral Components are stain with both dyes.

9. PS- Peripheral Smear 3.Staining of smear: Requirement: Giemsa stain, D/W OR buffer solution, staining rack, drying rack. Procedure: 1.Take 1 ml of D/W OR buffer solution in tube. 2.In this tube add 4-5 drops of Giemsa Stain. 3.Mix it well & pour on smear whole area. 4. Keep it for 15 mins than wash with water & keep it for air dry on drying rack.

10. PS- Peripheral Smear 4.Microscopic examination: 1. Observe the smear under the 45X OR 100X. 2. Examine the film by moving from one field to the next system optically and observe the WBC & RBC morphology.

11. PS- Peripheral Smear 2.Thick Smear: Thick smear is performing to check parasitic infection especially for Malaria parasite. Three major steps involve in Thick Smear. 1. Preparation of smear 2. Staining of smear 3. Observation

12. PS- Peripheral Smear 1.Preparation of smear. Requirement : Slide, applicator stick Sample: EDTA blood OR Capillary blood Procedure : 1. Take clean & dry slide. 2. Transfer drop of blood on slide & spread the blood drop in round or oval shape with applicator stick. 3. Keep it for air dry.

13. PS- Peripheral Smear 2.Staining of smear: Requirement: Giemsa stain, D/W OR buffer solution, staining rack, drying rack Procedure: 1. Take 1 ml of D/W in tube. 2. In this tube add 4-5 drops of Giemsa Stain. 3. Mix it well & pour on smear whole area. 4. Keep it for 15 mins than wash with water & keep it for air dry on drying rack.

14. PS- Peripheral Smear 3.Microscopic examination: Observe the smear under the 100x.