Raceway ponds - Mixotrophic and heterotrophic cultivation of microalgae _ ppt

Raceway ponds _ mixotrophic and hetrotrophic cultivation method, working process, application

1. VIVEKANANDHA ARTS AND SCIENCE COLLEGE FOR WOMEN VEERACHIPALAYAM,SANKGIRI,SALEM , TAMAIL NADU DEPARTMENT OF MICROBIOLOGY SUBJECT: MICROALGAL TECHNOLOGY SUBJECT INCHARGE : Dr. R. DINESHKUMAR, A SSISTANT PROFESSOR D EPARTMENT OF MICROBIOLOGY, VIAAS, SANGAKIRI. SUBMITTED BY: NANDHINI VENGADACHALAM, I MSC MICROBIOLOGY, DEPARTMENT OF MICROBIOLOGY, VIAAS, SANGAKIRI. TITLE; RACE WAY PONDS – HETEROTROPHIC AND MIXOTROPHIC CULTIVATION

2. CONTENT Raceway ponds Microalgae cultivation Methods Application Referance

3. Raceway ponds Race way pond wall thickness of 25 Cm. Pond length is 4.26m and the with is 1.8cm. Flowing water is diverted from natural streams or a well. It is the most common system large- scale outdoor cultivation of microalgae. Raceways are typically used for raising rainbow trout. To be considered a low risk method, waste must be treated and fish escapes prevented.

4. Race way pond working process A raceway pond circulates microalgae, nutrients, and water in a closed-loop channel using a paddlewheel to prevent sedimentation and distribute resources. This mixing is crucial for exposing algae to light and ensuring an equal distribution of nutrients and CO2, facilitating efficient growth.

5. Raceway pond application Raceway pond is outdoor cultivation method. The raceway pond application is a to commercial microalgae production (e.g. Spirulina , chlorella ). Nutraceuticals and food industry, biofuel production, pharmaceutical and cosmetics,waste water Treatment. Carbon sequenetration, aquaculture and agriculture is used .

6. Microalgae cultivation Microalgae cultivation is the process of growing microscopic algae in either marine of fresh water systems. Microalgae are primary producers in the oceans that convert water and carbon dioxide to biomass and oxygen in the presence of sunlight.

7. Microalgae cultivation methods Autotrophic cultivation – Sunlight + Co2 Heterotrophic cultivation – Dark room + organic carbon source Mixotrophic – Sunlight + Organic carbon source

8. Autotrophic Cultivation Autotrophic cultivation involves harnessing the photosynthesis capabilities of microalgae to produce biomass. There factors is crucial for maximizing biomass production. The optimizing autotrophic cultivation requires addressing factors such as light intensity carbon dioxide availability, nutrient balance and culture density. Organisms that create their own food from inorganic substances, like carbon dioxide and water, using energy from sunlight (photosynthesis) or chemical reactions (chemosynthesis). A microalgae cultivation system is a method for growing microalgae, which are tiny photosynthetic organisms, to produce biomass for various products like food, fuel, and pharmaceuticals.

9. Heterotrophic cultivation Heterotrophic cultivation is a method of growing microalgae in the presence of organic carbon source. The consumer in the food chain, synthesized to organic matter from inorganic source, performing cellular respiration to convert for food to usable energy for plant. Microalgae use photosynthesis to convert Co2 into biomass, heterotrophic cultivation provides an alternative way to maximize microalgae growth and lipid production

10. Fig: Heterotrophic cultivation

11. Heterotrophic cultivation working process Selected for organisms of microalgae (e.g. Chlorella vulgaris, schizochytrium) Culture medium prepared Sterilization Inoculation starter culture Fermentation /cultivation Biomass accumulation Downstream processing

12. Fig: Heterotrophic cultivation working process

13. Heterotrophic application Heterotrophic cultivation of microalgae has a wide range of potential application. Heterotrophic growth is high value products for bioactive compounds, lipids, protein. It can utilized for Industrial Biotechnology, Food and Beverage in dusty, Biofuel production , Nutraceuticals , Environmental and Agricultural used

14. Mixotrophic cultivation Mixotrophic cultivation involves providing microalgae with both light and organic carbon sources, such as glucose or acetate. This allows microalgae to efficiently utilize diverse carbon source, enhancing their growth rate and biomass productivity. Additionally, Mixotrophy enables microalgae to better adopt to fluctuating environmental conditions. Utilized to waste carbon streams making it a sustainable approach for bioproduction. This duel mode provides flexibility and often results in factor growth and higher biomass yield compared to purely autotrophic Or heterotrophic cultivation.

15. Mixotrophic cultivation working process The selection of organisms microalgae (e.g. Chlorella vulgaris , scenedesmus , spirulina , euglena gracilis ). Culture medium preparation Environmental condition – light source provided (Co2 photosynthesis, temperature, pH, aeration). Cultivation in bioreactor or open ponds (light +nutrients organic substrates provided). Growth and biomass accumulation Harvesting and processing.

16. Fig : Mixotrophic cultivation working process

17. Mixotrophic cultivation application Mixotrophic microalgae cultivation has diverse application in sustainable bio production. It can be utilized for the production of biofuels, food and nutraceuticals, waste water treatment, pharmaceuticals, agricultural used. The ability various carbon source mixotrophic open to new possibilities of economically viable bio production process.

18. Reference https://www.slideshare.net/slideshow/microalga e https://www.slideshare.net/slideshow/raceway-pondsppthttps : https://www.slideshare.net/slideshow/raceway-cultureppt

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