Ramachandran Plot

Ramachandran plot
Introduction
Diagram
Uses
Amino acid preference

1. Ramachandran Plot Dr. Ema SushanMinj Department of Microbiology

2. Introduction Ramachandran Plot is also called as Rama Plot ,Ramachandran diagram and ψφ Plot . It was originally developedin 1963 by G.N. Ramachandran, C. Ramakrishnan and V. Sasisekharan . It is a plot between psi and phi ψ ( Psi) shows the dihedral plot φ ( Phi) shows amino acid residues The ω angles of peptide bond is normally 180 ° , since the partial -double bonds character keeps the peptide planar. Because the dihedral values are circular and 0 ° is same as 360 ° , the edges of the Ramachandran Plot " wrap " Right to left and bottom to top.

3. Uses Showing conformations of psi and phi angles are possible for amino acid residues in a protein. Empirical distribution of datapoints observedin a single structure. Amino acid preference The effect of side chain is small. The major effect seen is that of the presence or absence of the methylene group at C - beta. Ex. Glycine has only a hydrogen atom for its side chain possess smaller van der Waals radius than CH3, CH2 or CH group (starts the side chain of all other amino acid)

4. Thank you