Scale up of animal cell cultture

 
Introduction

History

Scale up in suspension:Stirred culture,Continuous flow culture,Air- lift culture,Nasa bioreactor

Scale up in monolayer culture: Roller bottle culture , multisurface culture,fixed -bed culture

Other type of culture for scaling up: HARV Vessels,STLV vessels

Monitoring of scale up

Conclusion

References

1. Scale up of animal cell culture By KAUSHAL KUMAR SAHU Assistant Professor (Ad Hoc) Department of Biotechnology Govt. Digvijay Autonomous P. G. College Raj-Nandgaon ( C. G. )

2. Contents of the topic :   Introduction History Scale up in suspension: Stirred culture,Continuous flow culture,Air - lift culture,Nasa bioreactor Scale up in monolayer culture: Roller bottle culture , multisurface culture,fixed -bed culture Other type of culture for scaling up: HARV Vessels,STLV vessels Monitoring of scale up Conclusion References    

3. Introduction:

4. History: first description of an agitated, stainless steel animal cell culture bioreactor was one of 30 L for the production of inactivated foot and mouth disease vaccine (Telling and Elsworth 1965). However, recently, it has been reported that commercial bioreactors of 20,000 L at Lonza ( Hoeks et al. 2004) and Genentech (Meier 2005).

5.   (A )Scale up in suspension:   (1.)Stirred suspension culture:

6. (2.)Static suspension culture:   (3.)Continuous flow culture:

7. (4.)Air lift fermenter culture:

8.   (5.)NASA bioreactor:

9.     (B)Scale up in monolayer: (1.)Roller bottle culture:

10. (2.) Multisurface culture:

11. (3.) Microcarrier culture:

12.   (5.)Fluidized –bed reactors:

13.     (6.) Perfused monolayer culture:

14.   (7.)Hollow fiber culture:

15.   (8.)Ceramic matrix module:

16. (c)Other culture vessels for scale up: (1.) HARV Vessels (11) STLV Vessels

17.   (2.) Rotary Perfusion Culture System:

18. D)Monitoring of cell growth in scale up: (1.)In suspension cultures (2.)In monolayer culture:

19. Animal cell culture technique,M Clynes U.Satyanarayan (2005):Biotechnology Animal cell culture: Practical approach:R .W. Masters;oxford uni.press2000   References