SCALE-UP OF BIOREACTOR.pptx

SCALE-UP of Bioreactor in Fermentation.

1. SCALE-UP OF BIOREACTOR Prepared By Rahit Singha BMLT, MSc. Microbiology (Lynch, 2017)

2. INDEX Introduction Objective of scaling up When do we need scaling up studies Stages of scaling up studies Changes in operation of fermenters during scale up Conclusion References

3. INTRODUCTION Scale up in general is defined as increasing something in size, amount, or production. The essence of scale-up of a fermentation process is to demonstrate fermentation at large scale resulting in the same productivity and quality as that develop at small scale. (Allman, 2021)

4. OBJECTIVE OF SCALING UP To produce product of commercial value and to derive profit from the process. To increase the production capacity of a unit, thus lowers both investment costs and the variable costs. To lower or reduce the production cost. Scaling up production of Single Cell Protein from Spirulina Sp.

5. WHEN DO WE NEED SCALING UP STUDIES There is the need to do scaling ups in situation where we lack the basic operating data for the fermentation process. This occurs in the situation such as: New fermentation process Change in medium/strains of bacteria/fungi used New fermenters

6. STAGES OF SCALING UP STUDIES Primary screening: solid media studies Secondary screening (50ml – 1000ml) Laboratory scale fermenters (5L – 20L) Pilot scale fermenter (50L – 5000L) Industrial scale fermenter (>5000L) Industrial Scale (Marques MPC, 2017)

7. STAGES OF SCALING UP STUDIES Primary Screening Secondary Screening Lab Scale Fermenter Pilot Scale Fermenter Industrial Fermenter (Allman, 2021)

8. CHANGES IN THE OPERATION OF FERMENTERS DURING SCALE UP Power Bigger reactor needs more power Size and Volume of fermenter Changes in size and volume will affect Temperature & pH control system Heat Transfer Changes in size and complexity of huge fermenter vessel will affect sterilization process, heat removal process

9. CONCLUSION Scale-up is the process of expanding a fermentation process from a smaller-scale fermenter, where operational and production parameters have been studied, to a larger scale. However, engineers’ ultimate goal is to maximize production and efficiency in plant-scale production.

10. REFERENCES Marques MPC, Cabral JMS and Fernandes P. Bioprocess scale-up: quest for the parameters to be used as criterion to move from microreactors to lab-scale.  Journal of Chemical Technology and Biotechnology 2010; 85(9): 1184-1198 Xia J, Wang G, Lin J, Wang Y, Chu J, Zhuang Y and Zhang S. Advances and Practices of Bioprocess Scale-up. Adv Biochem Eng Biotechnol 2016; 152:137-51 Hsu YL, Wu WT. A novel approach for scaling-up a fermentation system. Biochem Eng J. 2002;11:123–30. Islam RS, Tisi D, Levy MS, Lye GJ. Scale-up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched kLa. Biotechnol Bioeng. 2008;99(5):1128–39. Tang YJ, Zhang W, Liu RS, Zhu LW, Zhong JJ. Scale-up study on the fed-batch fermentation of  Ganoderma lucidum  for the hyperproduction of ganoderic acid and  Ganoderma  polysaccharides. Process Biochem. 2011;46:404–8.

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