Screening for antibiotic producer organism from environmental sample.pptx

Screening for antibiotic producer organism from environmental sample

1. Screening for antibiotic producer organism from environmental sample

2. Screening for antibiotic-producing organisms from environmental samples involves the isolation and identification of microorganisms capable of producing antibiotics. Here's a general outline of the process: Sample Collection: Collect samples from the environment where you suspect antibiotic-producing organisms may be present. These samples can include soil, water, plant material, or other relevant sources. Dilution and Plating: Dilute the environmental sample and plate it on agar plates or other suitable culture media. Use selective media that encourage the growth of microorganisms with antibiotic production capabilities. This may involve adding specific nutrients or inhibitors to suppress the growth of non-target organisms.

3. Isolation of Pure Cultures: After incubation, observe the plates for colonies that appear distinct and different from others. Select individual colonies (representing different morphologies) and streak them onto fresh agar plates to obtain pure cultures. Pure cultures are important to ensure that the antibiotic activity observed is from a single organism. Primary Screening: Perform a primary screening to identify potential antibiotic producers. This can be done using methods such as the agar well diffusion assay. In this assay, the isolated organisms are streaked onto agar plates, and wells are made in the agar. The wells are filled with a susceptible indicator organism, and if the isolated organism produces antibiotics, it will inhibit the growth of the indicator organism around the well.

4. Secondary Screening: Further characterize the potential antibiotic producers obtained from the primary screening. This involves evaluating their antibiotic production capabilities using more specific assays, such as the Kirby-Bauer disc diffusion method or broth dilution methods (as described in the previous response). These assays help determine the spectrum of activity, potency, and minimum inhibitory concentration (MIC) of the antibiotics produced. Identification: Identify the antibiotic-producing organisms using techniques like microscopy, biochemical tests, and molecular methods (e.g., DNA sequencing). This step helps determine the taxonomic classification of the isolates, which can provide insights into the potential novel antibiotic-producing strains.

5. It's important to note that the screening process can be time-consuming and complex. It often requires specialized knowledge and expertise in microbiology and biochemistry. Additionally, appropriate safety precautions should be followed when handling environmental samples and potentially pathogenic microorganisms.