Screening models For immunosuppressants ..pptx

Explore the dynamic world of screening models for immunosuppressants in our comprehensive academic seminar. Delve into cutting-edge methodologies and advancements aimed at enhancing efficacy and safety profiles. Uncover the latest innovations driving the development of immunosuppressive therapies, vital for transplant success and autoimmune disorder management. Join us on this enlightening journey through the evolving landscape of immunosuppressant screening models.

1. IMMUNITY Preclinical Screening models for Immunosuppressive agents Guided By Mr. Mukul Tambe Assistant professor Dept. of Pharmacology 5/8/2024 1 Presented By Mr. Rahul Tipare M pharm (Pharmacology)

2. What are immunosuppressant ? How normal immune response occurs ? In-vivo preclinical screening model for immunosuppressant In-vitro preclinical screening model for immunosuppressants Summary References 5/8/2024 2 CONTENTS

3. IMMUNOSUPPRESSANTS Immunosuppressants are medications that suppress or dampen the activity of the immune system. APPLICATIONS ● Pr event the body from rejecting transplanted organs ● Treat autoimmune disorders where the immune system mistakenly attacks healthy tissues. Example = M ultiple sclerosis , Rheumatoid Arthritis Some existing immunosuppressants are Cyclosporine , tacrolimus , sirolimus , abatacept , belatacept 5/8/2024 Reference = Essential of medical pharmacology by K.D. Tripathi 3

4. Normal Immune Response & Site of action of Existing Immunosuppressants 5/8/2024 Reference = Essential of medical pharmacology by K.D. Tripathi & Speed pharmacology 4

5. in-vivo SCREENING MODELS FOR IMMUNOSUPPRESSENT DRUGS 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 5

6. Acute Systemic Anaphylaxis In Rats Female Sprague Dawley Rat Weight 120 gm Evaluation = shock symptoms and mortality counted 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 6

7. Modifications of Acute systemic anaphylaxis model 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 7

8. Guinea Pig Body weight 300 to 350 gm Anti- Anaphylactic Activity (Schultz Dale Reaction) Evaluation= The Contractions are Recorded with strain gauges by a Polygraph 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 8

9. Modifications of Anti Anaphylactic Activity ( Schultz Dale Reaction) 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 9

10. Passive Cutaneous Anaphylaxis Albino Rats Body weight 120 gm Evaluation = Amount of Evans blue that extracted from passive cutaneous anaphylactic reaction of control group compared to test group 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 10

11. Modifications of Passive Cutaneous Anaphylaxis model 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 11

12. MY BLOG Arthrus Type Immediate Hypersensitivity Wistar or Sprague Dawley Rat Body weight 220-280 gm Evaluation = The changes in footpad thickness is expressed as percent Change From the vehicle control group . 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 12

13. Modifications of Arthrus Type Immediate Hypersensitivity model 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 13

14. 123 Anywhere St. Any City, ST 12345 [email protected] [phone] Mailing Address Email Address Phone Number Delayed Type Hypersensitivity model Sprague Dawley Rat Body weight 150 – 230 mg 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 14

15. Modifications of Delayed Type Hypersensitivity model 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 15

16. In–vitro SCREENING MODELS FOR IMMUNOSUPPRESANTS 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 16

17. Inhibition of Histamine release from Mast cells 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 17

18. Inhibition of Histamine release from Mast cells 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 18

19. Chemiluminescence in Macrophages Chemiluminescence is a chemical reaction that produces light without the involvement of heat. 5/8/2024 Reference = Drug Discovery And Evaluation by H. Gerhard Vogal 19

20. 5/8/2024 20 SUMMARY Immunosuppressant and their Mechanism of action Need of immunosuppressants In-vivo models, different animals , drugs in different doses, molecules act as antigen In-vitro models, different methods by which we can study antigen antibody reaction outside body Basic difference in in-vivo, in-vitro, ex-vivo, techniques

21. 5/8/2024 21 REFERENCE Essential of Medical Pharmacology, by K.D. Tripathi. 2019. Jaypee The Health Sciences Publisher. New Delhi, London, Panama, Eighth Edition, Page no. 937-945. Drug Discovery and Evaluation Pharmacological Assays, by H. Gerhard Vogal . Springer publication. Berlin, Heidelberg, New York, Barcelona, Hong Kong, London. Second Completely Revised and Enlarged Edition, Page no. 788-805. https://youtu.be/vWh1AJwAIg0?si=kexgn3DNrtlTnuff (Speed Pharmacology Youtube Channel) Carvalho, V.F., Barreto, E.O., Diaz, B.L., Serra, M.F., Azevedo, V., Cordeiro, R.S., Martins, M.A. and e Silva, P.M., 2003. Systemic anaphylaxis is prevented in alloxan-diabetic rats by a mechanism dependent on glucocorticoids.  European journal of pharmacology ,  472 (3), pp.221-227. Baruah, C.C., Gupta, P.P., Nath, A., Patnaik, L.G. and Dhawan, B.N., 1998. Anti-allergic and anti-anaphylactic activity of picroliv —a standardised iridoid glycoside fraction ofpicrorhiza kurroa .  Pharmacological research ,  38 (6), pp.487-492 . Naik, S.R., Bhagat, S., Shah, P.D., Tare, A.A., Ingawale , D. and Wadekar , R.R., 2013. Evaluation of anti-allergic and anti-anaphylactic activity of ethanolic extract of Zizyphus jujuba fruits in rodents.  Revista Brasileira de Farmacognosia ,  23 (5), pp.811-818.

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