Seminar on pgr in plants

seminar on plant hormones.

1. Seminar on PLANT HORMONES AND GROWTH REGULATOR Presented by: Zamran Khan M.Sc 3 rd SEM The Oxford College Of Science

2. CONTENTS Introduction Classification Auxin Cytokinins Gibberellins Abscisic acid Ethylene Previously asked questions References

3. INTRODUCTION Plant growth regulators may be defined as any organic compounds, which are active at low concentrations in promoting, inhibiting or modifying growth and development. The term Hormone is derived from a Greek root ‘ hormao ’ which means ‘to stimulate’ ( Beylis and Starling, 1902). Thimann (1948) suggested using the term ‘ Phytohormone ’ for Hormones of plant. The naturally occurring growth substances are commonly known as plant hormones, while the synthetic ones are called growth regulator. Plant hormone is an organic compound synthesized in one part of the plant and translocated to another part, where in very low concentrations it causes a physiological response. The plant hormones are identified as promoters ( auxins, gibberellin and cytokinin ), inhibitors ( abscissic acid and ethylene ).

4. CLASSIFICATION There are five major plant growth regulators which includes : 1) Auxin 2) Cytokinins 3) Gibberellins 4) Abscisic acid (ABA) 5) Ethylene

5. AUXIN Derived from the Greek word " auxein " means- "to grow/increase ". It may be defined as a plant hormone which causes elongation of cells in shoots and is involved in regulating plant growth. Muslim physician  Ibn Tufail , as an early mention, wrote in his philosophical novel, that some flowers did seem to bend themselves towards light ,  alluding towards auxin . It was discovered by a German chemist Fritz Kogl and his co workers at the University of Utrecht in 1934. First plant hormone to be discovered. Indole acetic acid ( IAA ), is the naturally occuring Auxin

6. TYPES OF AUXINS Indole acids:   indole-3-propionic acid ( IPA )

7. Naphthalene acids Naphthalene acetic Acid (NAA) β- Naphthoxyacetic Acid (NOA)

8. Chlorophenoxy acids 2,4-Dichlorophenoxyacetic acid 2,4,5-Trichlorophenoxyacetic acid

9. Benzoic acids 2,4,6-Trichlorobenzoic acid 3,6-dichloro-2-methoxybenzoic acid

10. ROLE OF AUXIN Development of the embryo From the very first mitotic division of the zygote, gradients of auxin guide the patterning of the embryo into the parts that will become the organs of the plant. Apical dominance Growth of the shoot apex (terminal shoot) usually inhibits the development of the lateral buds on the stem beneath. This phenomenon is called apical dominance. Fruit development Pollination of the flowers of angiosperms initiates the formation of seeds. As the seeds mature, they release auxin to the surrounding flower parts, which develop into the fruit that covers the seeds

11. Plant B has apical bud removed so auxiliary buds grow. A B

12. Root initiation and development The localized accumulation of auxin in epidermal cells of the root initiates the formation of lateral or secondary roots. Auxin also stimulates the formation of adventitious roots in many species. Adventitious roots grow from stems or leaves rather than from the regular root system of the plant. Phototropism Plant bend towards unilateral light. This is due to higher concentration of auxin on the shaded side.

13. Evidence for the role of auxin in adventitious root formation With synthetic auxin Without synthetic auxin Adventitious roots growing from stem tissue Saintpaulia ( Gesneriaceae family) (African violets)

14. Phototropism

15. CYTOKININS This was discovered in the course of studies involved in identifying factors that stimulate plant cells to divide. Cytokinins  were discovered by Folke Skoog , Carlos Miller and co-workers in 1955. F. skoog discovered that degraded DNA after autoclaving was able to induce cell division in Tobacco pitch tissue. After thorough analysis of the degeaded product of DNA it was found that the active component was 6-fufuryl amino –purine (6-furfuryladenine) , and it was named as kinetin. The naturally occurring cytokinins are cis - trans- zeatin (ZEA), dihydrozeatin , isopentenyladenine (IPA) etc. The synthetic Cytokinins are kinetin, N-N diphenylurea , 6-benzylaminoprine(BAP) etc.

16. naturally occurring cytokinins

17. Synthetic Cytokinins

18. ROLE OF CYTOKININS Regulates the cell cycle/cell division ( hence,the name " cytokinins ) – especially by controlling the transition from G2 to mitosis. Control morphogenesis In plant tissue cultures, cytokinin is required for the growth of a callus (an undifferentiated, tumor-like mass of cells). Greening Promotes the light-induced formation of chlorophyll. Bud development Direct application of cytokinin promotes the growth of axillary buds.

19. Delay senescence senescence is the programmed aging process that occurs in plants (and other organisms for that matter). loss of chlorophyll, RNA, protein and lipids. cytokinin application to an intact leaf markedly reduces the extent and rate of chlorophyll and protein degradation and leaf drop. It delays the ageing of the plant.

20. Transgenic SAG12-IPT plant   Comparison of a transgenic SAG12-IPT plant with the wild type, Note the significantly delayed leaf senescence in the transgenic plant. Nicotiana ( Solanaceae family) (Tobacco plant)

21. GIBBERELLINS Gibberellins (GAs) were first isolated from the fungus Gibberella fujikuroi in 1926 by Japanese scientist E. Kurosawa G. fujikuroi causes baka’nae (foolish seedling) disease in rice, causing Excessive shoot elongation, Yellowish green leaves taller plants with absent or poorly developed grains Frequent lodging due to long stature Chemical was extracted & purified and named as Gibberellic Acid (GA). Now 80 different Gibberellins are available- GA1 to GA80 is available. The most commonly occurring gibberellins is GA3.

22. Gibberellic acid(GA 3 )

23. ROLE OF GIBBERELLINS Promotes stem elongation When applied to intact plants, GA usually causes an increase, unlike auxin. It overcomes dwarfism in mutants .

24. Overcomes dormancy in seeds Dormancy  is a period in an organism's life cycle when growth, development, and (in animals) physical activity are temporarily stopped. Gibberellins also have a fundamental role in breaking seed dormancy and stimulating germination. Many forms of dormancy are broken by GA. These include seed dormancy, dormancy of potato tubers and dormancy of shoot internodes and buds. GA can induce fruit enlargement External application of gibberellins can also enlarge fruit size in grapes

25. Sex expression In plants with separate male and female flowers, GA application can determine sex. For example, in cucumber and spinach, GA treatment increases the proportion of male flowers. In maize, GA treatment causes female flower development. Involved in parthenocarpic fruit development Development of fruit without fertilization . The fruit resembles a normal fruit, but it is seedless GA causes ovaries to mature without fertilization and produces bigger fruits.

26. Germination Gibberellins are involved in the natural process of germination. Before the photosynthetic apparatus develops sufficiently in the early stages of germination, the stored energy reserve of starch nourish the seedling. Flowering Exogenous GA application can induce flowering in species that ordinarily require cold treatment to bloom.

27. ABSCISIC ACID(ABA) ABA plays a major role in adaptation to abiotic environmental stresses, seed development, and germination. Abscisic acid is an important growth regulator for induction of embryogenesis. This is a growth inhibitor.

28. ROLE OF ABSCISIC ACID Seed Dormancy ABA plays a major role in seed dormancy During seed maturation, ABA levels increase dramatically. This inhibits germination and turns on the production of proteins that enable the embryo to survive dehydration during seed maturation.

29. ABA induces stomatal closure Solutes ( e.g. potassium and chloride ions) accumulate in guard cells causing water to accumulate in guard cells, making them turgid(swollen from water uptake.) ABA is one signal that causes guard cells to release solutes and thus release water, making them flaccid(compressed/shrink) and closing the stoma (pore) between them

30. Drought resistance Abscisic acid is the key internal signal that facilitates drought resistance in plants Under water stress conditions, ABA accumulates in leaves and causes stomata to close rapidly, reducing transpiration and preventing further water loss. ABA causes the opening of efflux K+ channels in guard cell plasma membranes, leading to a huge loss of this ion from the cytoplasm. The simultaneous osmotic loss of water leads to a decrease in guard cell’s turgidity, with consequent closure of stomata.

31. Inhibition of bud growth and shoot formation. Abscisic acid owes its names to its role in the abscission of plant leaves. In preparation for winter, ABA is produced in terminal buds. This slows plant growth and directs leaf primordia to develop scales to protect the dormant buds during the cold season.

32. ETHYLENE It is the only gaseous hormone of plants. It is produced naturally by higher plants and is able to diffuse readily, via intercellular spaces, throughout the entire plant body In 1934, Gane identified that plants could synthesise ethylene and in 1935 Crocker proposed ethylene to be the hormone responsible for fruit ripening and senescence of vegetative tissues. Apples and pears are examples of fruit that produce ethylene with ripening. Ethylene is responsible for the changes in texture, softening, color, and other processes involved in ripenining .

33. ROLE OF ETHYLENE Fruit ripening Under natural conditions, fruits undergo a series of changes, including changes in colour , declines in organic acid content and increases in sugar content. In many fruits, these metabolic processes often coincide with a period of increased respiration, the respiratory climacteric . During the climacteric there is also a dramatic increase in ethylene production. Ethylene can initiate the climacteric in a number of fruits and is used commercially to ripen tomatoes, avocados, melons, kiwi fruit and bananas.

34. Shoot Growth Applied ethylene has the capacity to influence shoot growth. Application of ethylene to dark-grown seedlings can cause reduced elongation of the stem, bending of the stem and swelling of the epicotyl or hypocotyl . Ethylene treatment of seedlings promotes hook closure and stem thickening rather than elongation. These etiolated pea seedlings were treated with 0, 0.1 and 1 ppm ethylene (left to right)

35. Flowering The ability of ethylene to affect flowering in pineapples has important commercial applications . Growth effects Inhibits logitudinal but promotes horizontal growth. Breaks dormancy

36. PREVIOUSLY ASKED QUESTIONS Absicic acid (3 marks , December 2014).

37. REFERENCES Chawla H.S(2009 ) , Introduction to plant biotechnology(3 rd edition), Science publishers, 22-23 Edwin F. George, Edwin F. George, Michael A. Hall, Geert -Jan De Klerk (2007) - Plant Propagation by Tissue Culture.The Background, Volume 1,Springer,175-282

38. Thank you