shalini microbiology presentation ppt.pptx

Microbiology

1. Microbiology Campylobacter Shalini Subban 223A

2. Campylobacter Campylobacter is a type of bacteria that can cause a diarrhea disease in people. Its name means “curved bacteria”, as the germ typically appears in a comma or “s” shape

3. Taxonomy 1. Domain: Bacteria
2. Kingdom: Eubacteria
3. Phylum: Proteobacteria 4. Class: Epsilonproteobacteria 5. Order: Campylobacterales 6. Family: Campylobacteriaceae 7. Genera: Campylobacter, Arcobacter 8.species: C. Jejuni ,C. Coli, C. lari

4. Morphology Gram-negative, curved, spiral-shaped, S-shaped rods
•Non- sporing • Motile (polar flagella)
• Oxidase positive
• Microaerophilic (grow best at 5% O2, 10% CO2, 85% N₂) at 42° C

5. Cultural properties 1. They are microaerophilic, best growth at 42°C in atmosphere containing 5% O2 & 10% CO2
2.They grow on a selective medium called Skirrow’s medium (containing lysed blood agar and vancomycin , polymyxin & trimethoprim).
3.Oxidase + ve 4. Catalase + ve 5. Nitrate Reducer
6. Thermophilic Grow at 42°C except C. Fetus (non thermophilic )

6. Antigenic Structure – Campylobacter
These bacilli have typical Gram negative antigenic structure with many different somatic “O” antigens and “H” flagellar antigens.

7. Pathogenicity Occurs with in 2-4 days(range of incubation period, 1-7 days) of ingestion of contaminated food, milk or water.
Cause acute gastroenteritis, last for days to several weeks, usually self limiting.
Infection first jejunum and ileum and later spread distally to affect terminal ileum, colon and rectum Virulence factor: Produce enterotoxin ,cytotoxin ,Adhesions Intracellular survival ,Ability to penetrate cells

8. Laboratory Diagnosis •Specimen: Stool (contain mucus, pus and blood), rectal swab, blood
•Culture: on selective media containing:
-Charcoal: to remove oxygen radicals
-Antibiotics: to inhibit other bacteria and enriched with blood
-Selective media:
1) Skirrow’s -blood agar
2) Campy-blood agar

9. Treatment -Usually self-limiting
-Patient require only fluids and electrolytes replacement and rehydration
-Antibiotics in case of septicemia : erythromycin

10. Thank you for your attention