Significance of shine dalgarno sequence

The shine dalgarno sequence is a ribosomal site in the prokaryotic bacterial mRNA which helps in protein synthesis by aligning the ribosome with the start codon. It's significance deals with it's effect and importance during the translation process within an mRNA.

1. Significance of shine Dalgarno SEQUENCE. Submitted By: Prajakta Panda 190704180001 B.Sc Biochemistry (4 th sem ) CUTM BBSR Campus

2. OVERVIEW ON TRANSLATION AND IT’S PROCESSES STEPS INCLUDED IN TRANSLATION INTRODUCTION TO SD SEQUENCE WHERE IT IS FOUND? DELETION OF SD SEQUENCE IT’S IMPORTANCE CONCLUSION

3. Translation is the process by which a protein is synthesized from the information contained in a molecule of messenger RNA (mRNA). During translation mRNA is read using the genetic code. The genetic code is a set of three letter combinations of nucleotides called codons. Codons are of different types. A start codon is used to mark the sites at which translation into protein sequence begins. The another stop codon is used to mark the site at which translation ends. Translation occurs at ribosome. The ribosome contains a large subunit (50S), a small subunit (30S), an entrance for tRNA molecules (A site), a site of elongation (P site) and an exit site for tRNAs (E site). Translation occurs in 3 stages: Initiation, Elongation, and Termination.

4. In bacteria, the small ribosomal subunit binds to the Shine-Dalgarno sequence to initiate translation. If the Shine-Dalgarno sequence is deleted, then translation initiation cannot take place, preventing protein synthesis.

5. Shine Dalgarno sequence is a sequence of 5 to 9 (typically 7) nucleotide preceding the start codon in prokaryotic mRNA that is recognized by the ribosome as the correct site for binding the mRNA molecule prior to start of translation. The sequence (AGGAGGU) binds a complementary sequence on the 16S ribosomal subunit, helping to form a stable complex between the ribosome and mRNA. The role of this sequence was first proposed by John Shine and Lynn Dalgarno.

6. It helps to initiate translation by binding the mRNA to the ribosome near the first AUG. It enables the initiation of protein synthesis by aligning the ribosome with start codon. It base pairs with the 16S rRNA. It can be on the end or it can be in the middle of an RNA.

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