Single Cell Protein - Slideshare PPT

Single Cell Protein -slideshare ppt

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1. Single Cell protein By- Priyabrata Karmakar Couse – Project & Seminar I MSc Microbiology (1 st sem ) JIS UNIVERSITY

2. Contains- Brief description History Used Microorganisms as raw material Production Places Production Steps Uses of SCP Conclusion R eferences

3. WHAT ARE SINGLE CELL PROTEINS ? SCP are dried cells of microorganisms which can be used as protein-rich food supplements by humans and animals They are used as animal feed & can be used for human feed as protein supplement . Also called ‘ Novel Food ’ & ‘ Minifood ’.

4. History of Scp In 1950’s British Petroleum initiated production of SCP on commercial basis . Pruteen was the 1st commercial SCP used as animal feed additive Pruteen was produced from bacteria Methylophilus methylotrophus cultured on methanol & had 72 % protein content. Earlier known as ‘ Microbial Protein ’. In1966 M.I.T Professor Carroll L.Wilson renamed it as “ Single Cell Proteins ”

5. micro-organisms used for SCP production Fungi Aspergillus fumigatus Aspergillus niger Rhizopus cyclopium Yeast Saccharomyces cerevisae Candida tropicalis Candida utilis Algae Spirulina sps . Chlorella pyrenoidosa Chondrus crispus Bacteria Pseudomonas fluroescens Lactobacillus Bacillus megaterium

6. SCP PRODUCTION IN INDIA National Botanical Research Institute (NBRI ). Central Food Technological Research Institute (CFTRI ) In CFTRI, SCP is produced from algae cultured on sewage

7. Production of SCP Selection of Suitable Strain Fermentation Harvesting Post harvest treatment Processing of SCP for food Single Cell Protein production involves following steps:

8. Step- 1 Selection of Suitable Strain It a very critical step as the quality of protein depends totally on the microbe that is used for the production. Thus careful selection of the strain should be done. Care should be taken that the selected strain should not produce any toxic or undesirable effects in the consumer.

9. Step -2 Fermentation Is done in a large chamber either of glass or stainless steel called “ Fermentor ” Fermentation should be done under sterilized conditions Controlled conditions as necessary e.g. Temperature , Pressure , pH , Humidity etc Usually fed-batch cultures are used for the fermentation of microbes

10. Step -3 Harvesting When the colonies of microbes are fully developed, they are then harvested The bulk of cells are removed from the fermenter by decantation .

11. Step -4 Post Harvesting After harvesting, the cells are subjected to a variety of processes. Post harvesting treatments includes steps like separation by centrifugation , washing , drying , etc.

12. Step -5 Final processing of ScP Pure protein isolation can be done by disrupting the cell wall through crushing , crumbling, cycles of freezing & thawing, grinding & thermal shocks. Nucleic acid can be remove by- By treatment with Nacl 10% By Chemicals e.g. NaoH Thermal shocks Enzymes Treatment e.g. ribonucleases

13. Uses of SCP As protein supplemented food -source of vitamins , amino acids , minerals, crude fibers , etc As health food - controls obesity , provides instant energy Therapeutic and natural medicines - reduce body weight , cholestero l, stress , lowers blood sugar level in diabetic In cosmetics -important role in maintaining healthy hair (vitamin A and B), many herbal beauty products ( biolipstics and herbal face cream) Poultry and cattle feed - excellent, convenient source of protein and other nutrients

14. CONCLUSION At present SCP production is in its infancy. One of the ways to enhance productivity and quality is genetic improvements of micro-organisms. Using microbial biomass as a food source deserves serious consideration because of insufficient world food supply and high protein content of most microorganisms. Microbes have shorter generation time , allow easy transformation, utilize many substrates, have no requirements in arable lands or any particular season to grow and have the possibility of continuous production in any part of the world .

15. REFERENCES https://en.wikipedia.org/wiki/Single-cell_protein https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/single-cell-protein https://pubmed.ncbi.nlm.nih.gov/8543324/ https://www2.slideshare.net/FIRDOUS88/single-cell-protein https://www.biologydiscussion.com/single-cell-protein/production-of-single-cell-protein-and-mushrooms/10392 Thank You