single component.pptx

PHARMACEUTICAL ANALYSIS: UV SPECTROSCOPY- SINGLE COMPONENT ANALYSIS

1. QUANTITATIVE ANALYSIS METHODS IN UV VISIBLE SPECTROSCOPY: SINGLE COMPONENT ANALYSIS METHODS 1 PRESENTED BY , Anjana P Dinesh M Pharm College Of Pharmaceutical Sciences, Government Medical College, Kannur

2. UV VISIBLE SPECTROSCOPY 2 Fig 1:UV Spectroscopy

3. 3 Single-component pharmaceutical assays can be determined using single-point standardization, which involves  measuring the absorbance spectrum of a standard solution of the reference sample and a sample solution . The concentration of the absorbing substance is mainly calculated by using 3 methods: SINGLE-COMPONENT ANALYSIS METHODS Standard absorptivity method Calibration graph Single or double-point standardization

4. 4 STANDARD ABSORPTIVITY VALUE The concentration of an unknown compound can be determined by using the standard absorptivity value and the measured absorbance. Mainly used for stable substances – broad absorption bands. This method is used when the substance is expensive or difficult to obtain. A   = A 1% 1   cm bc A= absorbance of the sample A 1% 1   cm = standard absorptivity b= path length C= concentration of a substance

5. 5 CALIBRATION GRAPH METHOD The absorbances of a number of standard solutions of the reference substance at the concentration of the sample solutions are measured and a calibration graph is drawn. Fig 2:Calibration Graph

6. 6 SINGLE OR DOUBLE-POINT STANDARDISATION This method involves the measurement of the absorbance of a sample solution and of a standard solution of the reference substance. The concentration of a substance in the sample is measured from the proportional relationship between the absorbance and concentration. C test = A test x C std A std C test =concentration of sample A test =absorbance of sample C std =concentration of standard A std =absorbance of standard

7. 7 REFERENCES 1.Practical pharmaceutical chemistry;4 th edition-part two;A.H Beckett,J B Stenlake;pg no:278-281. 2.Principles of instrumental analysis;6 th edition ; Holler,Skoog,Grouch;pg no:369-375. 3. https:// www.slideshare.net/DivyaV44/uvvisible-spectroscopy. 4. https:// www.slideshare.net/suasini/uv-and-visible-appications . 5. wikimedia.org/wiki/ File:Schematic_of_UV-_visible_spectrophotometer.png

8. 8 Thank you