SIWES PRESENTATION ON MICROBIOLOGY DEPT.pptx

A report on internship in a laboratory

1. AN ORAL PRESENTATION ON THE STUDENT INDUSTRIAL WORK EXPERIENCE SCHEME [SIWES] UNDERTAKEN AT MICROBIOLOGY DEPARTMENT, FEDERAL POLYTECHNIC EDE OSUN STATE BY AKINLOYE OLUWATOYIN PRAISE 20191222 MICROBIOLOGY DEPARTMENT FEDERAL UNIVERSITY OF AGRICULTURE, ABEOKUTA.

2. OUTLINE 1. TITLE 2. AIM 3. MATERIALS/STAINS USED 4. THEORY 5. PROCEDURE 6. OBSERVATION 7. PRECAUTION 8. CONCLUSION

3. INTRODUCTION The Ubiquitous nature of microorganisms have called for constant researches to know if a sample or food is healthy or not or if the body is sick or not. TITLE: THICK AND THIN BLOOD FOR MALARIA PARASITE AIM To identify malaria parasite in the blood. MATERIALS/STAIN USED 1. Small quantity of blood 2. Slides 3. Sterile needles 4. Giesma stain 5. Lieshman stain 6. Microscope

4. THEORY : Thick blood smears are most useful for detecting the presence of parasite, because they examine a large of sample of blood. Often there are few parasite in the blood at the time the test is done. A thin blood smear is a drop of blood that is spread across a large area of the slide. PROCEDURE : A patient blood sample was collected and taken to the laboratory inside anti-coagulant bottle. PROCEDURE FOR THIN FILM A drop of blood out of the patients sample was dropped with aid of sterile pipette on the edge of a clean grease free sterile slide. A cover slip was placed on it at an angle of 90 degrees and it was dragged to make a thin film. STAINING FOR THE THIN FILM Undiluted Lieshman stain wad added directly to the smear. After fixation for one minute, the stain was diluted in 1:2 with neutral or slightly alkaline phosphate buffer pH 7-7.2 and stained for 10 minutes. It was observed with x100 immersion objective.

5. PROCEDURE FOR THICK FILM To make a thick film, two or three drops of fresh blood with anti-coagulant was placed on a sterile clean slide. The drop was mixed in a circular motion over an area of about 2 centimeters in diameter. It was mixed continuously for about 30 seconds to prevent formation of fibrin strands that may obscure parasites after staining. STAINING FOR THICK FILM The film was immersed in 0.5% aqueous methylene blue [for one second]. It was rinsed briefly and gently by dipping it into water or holding it under tap water slowly. The slide face was placed downwards in Giesma stain. [Field stain A and B can be used in the absence of Giesma stain]. The slide was removed from the stain and washed by flooding it briefly under a tap for 1 second. The slide was placed in an upright position to drain and dry. It was observed with x100 oil immersion objective.

6. OBSERVATION : It was observed that the chromatin of parasite stains dark red which indicates the presence of plasmodium species i.e malaria parasite It was observed that the cytoplasm of parasite stains blue, while the schuffer dots stained red and the maurer dots stains red mave . MALARIA PARASITE COLOUR Chromatin of parasite Dark red Cytoplasm of parasite Blue Schuffer dots Red Maurer dots Red mave

7. PRECAUTIONS It was ensured that anti-coagulant bottle was used to collect the sample for thin film. It was ensured that the blood sample was mixed properly to avoid formation of fibrin strands. It was ensured that staining of both films was done properly. CONCLUSION At the end of the practical, malaria parasite was detected using the thick and thin blood technique.