Snapgene

it is a software which helps the molecular biologist nd biotechnologist to plan their experimnets.

1. S NAPGENE View richly annotated sequence files Manish Thakur Systems Biology and Bioinformatics M.Sc-1

2. OUTLINE Introduction WHAT S napgene do visualize annotate Plan & DOCUMENT Strategies followed Output Conclusions

3. SnapGene  offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures. The streamlined interface supports a range of cloning and PCR manipulations. SnapGene was developed by GSL Biotech Chicago . First version of SnapGene was 0.2 released on Apr 3, 2008. Latest version available SnapGene 2.7.1 on Mar 6, 2014. Buy/Free trial of the software from the SnapGene website www.snapgene.com. INTRODUCTION

4. SYSTEM REQUIREMENTS O.S Windows 8, 7, Vista or XP Mac OS X 10.7 or later Memory 1 GB RAM Hard Disk 250 MB available disk space Display 1024 x 768 or higher resolution Written in Visual Studio, C++

5. • Create a DNA sequence file. • Browse or print a DNA sequence and its annotations using customizable Map, Sequence, Enzymes, Features, Primers, and History views. • Search a DNA sequence to match either a DNA query, or a protein translation, or an annotation. • Open and edit sequence trace files, and align them to a reference sequence. VISUALIZE

6. • Automatically annotate common features, or manually annotate coding sequences and other features. •Design and annotate primers for PCR, sequencing, or mutagenesis. •Identify open reading frames (ORFs) with a single mouse click. ANNOTATE

7. Simulate restriction cloning and other types of cloning using a rich intuitive interface. •Simulate conventional or overlap extension PCR. •Simulate primer-directed mutagenesis. •Simulate agarose gel electrophoresis. •Record all of your manipulations in an automatically generated graphical cloning history. PLAN AND DOCUMENT

8. What we can do with SnapGene Restriction Cloning Primers & PCR Mutagenesis Gibson Assembly In-Fusion Cloning Gateway Cloning TA or GC cloning

9. Restriction Cloning Cloning by restriction enzyme digestion and ligation is a simple and easy way of moving a fragment of double stranded DNA from one plasmid to another .

10. PRIMER DESIGNING & PCR   Amplify  a single copy or a few copies of a piece of  DNA .

11. PCR Machine Restriction Cloning

12. SnapGene has a specific purpose and it is highly flexible as far as finding items or groups of similar items. Its applicability is restricted to the field of molecular biology. I perform restriction cloning ,Primers & PCR strategy under SnapGene It is an indispensable tool for building plasmid maps of our various recombinant constructs to aid with the cloning process. It is a user-friendly & time saver software for RDT projects. CONCLUSION

13. Every DNA construct in a lab will be fully documented . Publications will include complete annotated sequences and histories of every DNA construct used . Time and money will be saved, and biomedical research will move faster. Vision of the Future with SnapGene