Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology ||

Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry

*images use in this ppt is only for educational purpose
In this presentation, i tell about substrate level phosphorylation
Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)

1. Substrate Level Phosphorylation And Its Mechanism Name : Darshan Ratan Jadhav Class : F.Y.B.Pharmacy Subject : Biochemistry College : K.K.Wagh College of Pharmacy, Nashik

2. Content Introduction Example Reaction Reference Content Introduction Mechanism The role of substrate-level phosphorylation in aerobic respiration. Refference

3. Substrate Level Phosphorylation ➢ Phosphorylation involves the transfer of phosphate group from one compound to other. ➢ Substrate level phosphorylation is a direct phosphorylation of ADP with a phosphatase group by using the energy obtain from a coupled reaction. ➢ Occurs in cytoplasm ( glycolysis – due to aerobic and anaerobic condition) and in mitochondrial matrix ( krebs cycle – anaerobic condition)

4. Genral Substrate Level Phosphorylation Reaction

5. Mechanism of Substrate level phosphorylation • High-energy phosphate groups are transferred directly from phosphorylated substrates to ADP • ATP is synthesized via substrate-level phosphorylation in glycolysis and the Krebs cycle

6. Catalysis

7. The role of substrate-level phosphorylation in aerobic respiration Substrate-level Phosphorylation and Aerobic Respiration: We know that there is the formation of 32 ATP molecules in total (since 2 ATP molecules are used in the beginning of glycolysis the final ATP yield is 30) as a result of the complete oxidation of one glucose molecule in aerobic respiration. Out of all 32 ATP molecules, 26 are produced by way of oxidative phosphorylation with the involvement of NADH and FADH2, whereas the remaining 6 are the result of substrate-level phosphorylation.

8. During glycolysis, the enzyme phosphoglycerate kinase transfers the high energy phosphoryal group from the carboxyl group of 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate .

9. during glycolysis, the enzyme pyruvate kinase transfers the high energy phosphoryl group from phosphoenolpyruvate to ADP, forming ATP and pyruvate. 2. ➢ In glycolysis during substrate level phosphorylation 4 ATP molecules are produce .

10. During TCA cycle (Citric acid cycle; Kreb’s cycle), the enzyme succinyl-CoA synthetase breaks the thioester bond of succinyl-CoA andthe energy released in this breakage is used to drive the synthesis of a phosphoanhydride bond in GTP or ATP. Succinate is the product in the process. 3. ➢ In This Step 2 ATP is produce

11. Refference Biochemistry Book By U. Satyanarayana & U. Chakrapani. www.google.com Guidance of subject teacher